Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/62340

TitleEvaluation of tenogenic differentiation potential of selected subpopulations of human adipose‐derived stem cells
Author(s)Gonçalves, A I
Berdecka, Dominika
Rodrigues, Márcia T.
Eren, Aysegul Dede
Boer, Jan de
Reis, R. L.
Gomes, Manuela E.
KeywordsAdipose derived stromal/stem cells
Subpopulation
Tenogenic differentiation
Tenomodulin
Transforming growth factor beta 3
Issue dateOct-2019
PublisherWiley
JournalJournal of Tissue Engineering and Regenerative Medicine
CitationGonçalves A. I., Berdecka D., Rodrigues M. T., Eren A. D., Boer J., Reis R. L., Gomes M. E. Evaluation of tenogenic differentiation potential of selected subpopulations of human adipose†derived stem cells, J Tissue Eng Regen Med., pp. 1-14, doi:10.1002/term.2967, 2019
Abstract(s)Identification of a suitable cell source and bioactive agents guiding cell differentiationtowards tenogenic phenotype represents a prerequisite for advancement of cellâ based therapies for tendon repair. Human adiposeâ derived stem cells (hASCs) are apromising, yet intrinsically heterogenous population with diversified differentiationcapacities. In this work, we investigated antigenicallyâ defined subsets of hASCsexpressing markers related to tendon phenotype or associated with pluripotency thatmight be more prone to tenogenic differentiation, when compared to unsortedhASCs. Subpopulations positive for tenomodulin (TNMD+ hASCs) and stage specificearly antigen 4 (SSEAâ 4+ hASCs), as well as unsorted ASCs were cultured up to 21days in basic medium or media supplemented with TGFâ β3 (10 ng/ml), or GDFâ 5(50 ng/ml). Cell response was evaluated by analysis of expression of tendonâ relatedmarkers at gene level and protein level by real time RTâ PCR, western blot, and immu-nocytochemistry. A significant upregulation of scleraxis was observed for both sub-populations and unsorted hASCs in the presence of TGFâ β3. More prominentalterations in gene expression profile in response to TGFâ β3 were observed forTNMD+ hASCs. Subpopulations evidenced an increased collagen III and TNC deposi-tion in basal medium conditions in comparison with unsorted hASCs. In the particularcase of TNMD+ hASCs, GDFâ 5 seems to influence more the deposition of TNC.Within hASCs populations, discrete subsets could be distinguished offering variedsensitivity to specific biochemical stimulation leading to differential expression oftenogenic components suggesting that cell subsets may have distinctive roles in thecomplex biological responses leading to tenogenic commitment to be further exploredin cell based strategies for tendon tissues.
TypeArticle
URIhttp://hdl.handle.net/1822/62340
DOI10.1002/term.2967
ISSN1932-6254
e-ISSN1932-7005
Publisher versionhttps://onlinelibrary.wiley.com/doi/pdf/10.1002/term.2967
Peer-Reviewedyes
AccessOpen access
Appears in Collections:3B’s - Artigos em revistas/Papers in scientific journals

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