Utilize este identificador para referenciar este registo: https://hdl.handle.net/1822/62104

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dc.contributor.authorGranja, Sara Costapor
dc.contributor.authorMarchiq, Ibtissampor
dc.contributor.authorBaltazar, Fátimapor
dc.contributor.authorPouysségur, Jacquespor
dc.date.accessioned2019-11-14T11:18:45Z-
dc.date.issued2014-
dc.identifier.isbn978-1-4939-0855-4por
dc.identifier.issn1064-3745-
dc.identifier.urihttps://hdl.handle.net/1822/62104-
dc.description.abstractZinc finger nucleases are reagents that induce DNA double-strand breaks at specific sites that can be repaired by nonhomologous end joining, inducing alterations in the genome. This strategy has enabled highly efficient gene disruption in numerous cell types and model organisms opening a door for new therapeutic applications. Here, we describe the disruption of CD147/basigin by this technique in a human cancer cell line.por
dc.language.isoengpor
dc.publisherHumana Presspor
dc.rightsclosedAccesspor
dc.subjectBasiginpor
dc.subjectCell Line, Tumorpor
dc.subjectDeoxyribonuclease Ipor
dc.subjectFlow Cytometrypor
dc.subjectGene Knockout Techniquespor
dc.subjectHumanspor
dc.subjectPlasmidspor
dc.subjectTransfectionpor
dc.subjectTransformation, Geneticpor
dc.subjectZinc Fingerspor
dc.subjectDNA double-strand breakspor
dc.subjectGene targetingpor
dc.subjectKnockoutpor
dc.subjectZinc finger nucleasepor
dc.titleGene disruption using zinc finger nuclease technologypor
dc.typebookPartpor
oaire.citationStartPage253por
oaire.citationEndPage260por
oaire.citationVolume1165por
dc.identifier.eissn1940-6029-
dc.identifier.doi10.1007/978-1-4939-0856-1_17por
dc.date.embargo10000-01-01-
dc.identifier.eisbn978-1-4939-0856-1por
dc.subject.wosScience & Technologypor
sdum.journalMethods in Molecular Biology-
sdum.conferencePublicationCANCER CELL SIGNALING: METHODS AND PROTOCOLS, SECOND EDITIONpor
sdum.bookTitleCancer cell signalingpor
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