Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/62104

TitleGene disruption using zinc finger nuclease technology
Author(s)Granja, Sara Costa
Marchiq, Ibtissam
Baltazar, Fátima
Pouysségur, Jacques
KeywordsBasigin
Cell Line, Tumor
Deoxyribonuclease I
Flow Cytometry
Gene Knockout Techniques
Humans
Plasmids
Transfection
Transformation, Genetic
Zinc Fingers
DNA double-strand breaks
Gene targeting
Knockout
Zinc finger nuclease
Issue date2014
PublisherHumana Press
JournalMethods in Molecular Biology
Abstract(s)Zinc finger nucleases are reagents that induce DNA double-strand breaks at specific sites that can be repaired by nonhomologous end joining, inducing alterations in the genome. This strategy has enabled highly efficient gene disruption in numerous cell types and model organisms opening a door for new therapeutic applications. Here, we describe the disruption of CD147/basigin by this technique in a human cancer cell line.
TypeBook part
URIhttp://hdl.handle.net/1822/62104
ISBN978-1-4939-0855-4
e-ISBN978-1-4939-0856-1
DOI10.1007/978-1-4939-0856-1_17
ISSN1064-3745
e-ISSN1940-6029
AccessRestricted access (Author)
Appears in Collections:ICVS - Livros e Capítulos de Livros

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