Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/61934

TitlePraziquantel-solid lipid nanoparticles produced by supercritical carbon dioxide extraction: physicochemical characterization, release profile, and cytotoxicity
Author(s)Andrade, Luciana N.
Oliveira, Daniele M. L.
Chaud, Marco V.
Alves, Thais F. R.
Nery, Marcelo
da Silva, Classius F.
Gonsalves, Joyce K. C.
Nunes, Rogéria S.
Corrêa, Cristiane B.
Amaral, Ricardo G.
Sanchez-Lopez, Elena
Souto, Eliana
Severino, Patrícia
Keywordspraziquantel
solid lipid nanoparticles
supercritical fluid
fibroblasts cell line
Issue date28-Oct-2019
PublisherMDPI
JournalMolecules
CitationAndrade, Luciana N.; Oliveira, Daniele M. L.; Chaud, Marco V.; Alves, Thais F. R.; Nery, Marcelo; da Silva, Classius F.; Gonsalves, Joyce K. C.; Nunes, Rogéria S.; Corrêa, Cristiane B.; Amaral, Ricardo G.; Sanchez-Lopez, Elena; Souto, Eliana; Severino, Patrícia, Praziquantel-solid lipid nanoparticles produced by supercritical carbon dioxide extraction: physicochemical characterization, release profile, and cytotoxicity. Molecules, 24(21), 3881, 2019
Abstract(s)Solid lipid nanoparticles (SLNs) can be produced by various methods, but most of them are difficult to scale up. Supercritical fluid (SCF) is an important tool to produce micro/nanoparticles with a narrow size distribution and high encapsulation efficiency. The aim of this work was to produce cetyl palmitate SLNs using SCF to be loaded with praziquantel (PZQ) as an insoluble model drug. The mean particle size (nm), polydispersity index (PdI), zeta potential, and encapsulation efficiency (EE) were determined on the freshly prepared samples, which were also subject of Differential Scanning Calorimetry (DSC), Fourier-Transform Infrared Spectroscopy (FTIR), drug release profile, and in vitro cytotoxicity analyses. PZQ-SLN exhibited a mean size of ~25 nm, PdI ~ 0.5, zeta potential ~−28 mV, and EE 88.37%. The DSC analysis demonstrated that SCF reduced the crystallinity of cetyl palmitate and favored the loading of PZQ into the lipid matrices. No chemical interaction between the PZQ and cetyl palmitate was revealed by FTIR analysis, while the release or PZQ from SLN followed the Weibull model. PZQ-SLN showed low cytotoxicity against fibroblasts cell lines. This study demonstrates that SCF may be a suitable scale-up procedure for the production of SLN, which have shown to be an appropriate carrier for PZQ.
TypeArticle
URIhttp://hdl.handle.net/1822/61934
DOI10.3390/molecules24213881
ISSN1420-3049
Publisher versionhttps://www.mdpi.com/journal/molecules
Peer-Reviewedyes
AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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