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TitleSynthesis and characterization of fructosyltransferase from Aspergillus oryzae ipt-301 for high fructooligosaccharides production
Author(s)Cunha, Josivan S.
Ottoni, Cristiane A.
Morales, Sergio A. V.
Silva, Elda S.
Maiorano, Alfredo E.
Perna, Rafael F.
KeywordsAspergillus oryzae IPT-301
Submerged fermentation
Enzymatic characterization
Issue dateJun-2019
PublisherBrazilian Society of Chemical Engineering
JournalBrazilian Journal of Chemical Engineering
CitationCunha, Josivan S.; Ottoni, Cristiane A.; Morales, Sergio A. V.; Silva, Elda S.; Maiorano, Alfredo E.; Perna, Rafael F., Synthesis and characterization of fructosyltransferase from Aspergillus oryzae ipt-301 for high fructooligosaccharides production. Brazilian Journal of Chemical Engineering, 36(2), 657-668, 2019
Abstract(s)Fructooligosaccharides (FOS) are mainly produced by microbial fructosyltransferases (FTase, E.C., and Aspergillus oryzae IPT-301 has shown high fructosyl transferring and low hydrolytic activities, which leads to high FOS production yields, but the main operating parameters for its best performance have been scarcely studied. Thus, this work aimed to evaluate the cellular growth, production and characterization of mycelial and extracellular FTases by Aspergillus oryzae IPT-301. Experimental design showed that the extracellular FTase performance was optimized (high transfructosylation activity and low hydrolytic activity) for reaction pH 5.5 - 6.75 and temperature of 45-50 °C and was fitted by the Michaelis-Menten model, while the mycelial FTase showed better performance at pH below 6.5 and temperature above 46 °C and was better fitted by the Hill model. The results obtained showed that the fungus represents a promising source for FOS production on a laboratorial scale.
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AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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