Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/61666

TitleProduction and bioengineering of recombinant pharmaceuticals
Author(s)Aguiar, Tatiana Quinta
Santos, Sílvio Roberto Branco
Martins, Ivone M.
Domingues, Lucília
Oliveira, Carla Cristina Marques de
KeywordsPhage display
Antimicrobial peptides
Endolysins
Lectins
Recombinant proteins
Heterologous expression hosts
Issue date2019
PublisherAcademic Press
CitationAguiar, Tatiana Q.; Santos, Sílvio B.; Martins, Ivone M.; Domingues, Lucília; Oliveira, Carla, Chapter 9 - Production and bioengineering of recombinant pharmaceuticals. Proteins: Sustainable Source, Processing and Applications, Academic Press, 2019. ISBN: 978-0-12-816695-6, 259-293
Abstract(s)Most therapeutic proteins on the market are obtained recombinantly from well-established production platforms. Furthermore, hundreds of recombinant proteins are already in clinical trials for the therapy of cancers, infections, and other diseases. Recent advances in the tools and strategies used in microbial production and purification processes have been highly beneficial for developing new bioproducts, and thus for further evolving the biopharmaceutical industry. Due to their simplicity, high-yield, and scalability, microbial-based expression systems, mainly the bacterium Escherichia coli and the yeast Pichia pastoris, have been widely explored for producing and bioengineering recombinant proteins and peptides for biomedical applications. The immense diversity of research studies on this subject anticipates a sharp increase of novel recombinant pharmaceuticals. This chapter focuses on recombinant approaches used for obtaining Phage Display-derived proteins/peptides, antimicrobial peptides (AMPs), phage-encoded endolysin enzymes, and lectins, which were selected as case studies. AMPs and endolysins have emerged in recent years as effective alternatives to conventional antibiotics. On the other hand, lectins have well-demonstrated potential as anticancer agents and as antimicrobial/viral tools. A succinct overview on the main production and purification strategies employed for each case, along with the latest advances in producing hosts, will be presented. In addition, the application of the Phage Display technique for identifying new therapeutic protein/peptides and their potential uses in the health field will be highlighted.
TypeBook part
URIhttp://hdl.handle.net/1822/61666
ISBN978-0-12-816695-6
DOI10.1016/B978-0-12-816695-6.00009-X
Publisher versionhttps://www.sciencedirect.com/science/article/pii/B978012816695600009X
Peer-Reviewedyes
AccessRestricted access (UMinho)
Appears in Collections:CEB - Livros e Capítulos de Livros / Books and Book Chapters

Files in This Item:
File Description SizeFormat 
document_51775_1.pdf
  Restricted access
1,53 MBAdobe PDFView/Open

Partilhe no FacebookPartilhe no TwitterPartilhe no DeliciousPartilhe no LinkedInPartilhe no DiggAdicionar ao Google BookmarksPartilhe no MySpacePartilhe no Orkut
Exporte no formato BibTex mendeley Exporte no formato Endnote Adicione ao seu ORCID