Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/61625

TitleEffects of altered corticosterold milieu on rat hippocampal neurochemistry and structure - An in vivo magnetic resonance spectroscopy and imaging study
Author(s)Schubert, Mirjam I.
Kalisch, Raffael
Sotiropoulos, Ioannis
Catania, Caterina
Sousa, Nuno
Almeida, Osborne F. X.
Auer, Dorothee P.
KeywordsAnimals
Aspartic Acid
Atrophy
Choline
Creatine
Dexamethasone
Energy Metabolism
Glucocorticoids
Glutamic Acid
Hippocampus
Hydrocortisone
Inositol
Male
Multivariate Analysis
Rats
Rats, Wistar
Image Processing, Computer-Assisted
Magnetic Resonance Imaging
Magnetic Resonance Spectroscopy
Corticosterone
Glutamate
Proton spectroscopy
magnetic resonance
Issue dateSep-2008
PublisherElsevier
JournalJournal of Psychiatric Research
Abstract(s)Altered corticosteroid milieu induces changes in hippocampal volume, neuronal structure, neurochemistry and cognitive function in humans and rodents. This in vivo magnetic resonance spectroscopy (1H MRS) and imaging (MRI) study investigated whether long-term alterations of the corticosteroid milieu cause: (i) metabolic and/or (ii) structural changes of the rat hippocampus. Therefore, hypocortisolism was induced by adrenalectomy (ADX), normocortisolism by ADX with low-dose corticosterone replacement, and hypercortisolism by ADX and high-dose dexamethasone treatment (for 11 weeks, respectively). All groups including a control group (n=23) were studied by in vivo 1H MRS and MR volumetry. Effects of treatment on normalized hippocampal metabolites and volumes were tested for significance using one-factorial multivariate analysis of variance (MANOVA). Hypercortisolemic rats revealed significantly elevated glutamate. Hypocortisolemic rats showed significantly decreased myo-inositol ratio levels, and were associated with significantly reduced normalized hippocampal volumes. Our findings suggest chronic hypercortisolism to be associated with glutamate-mediated excitotoxicity in the absence of volumetric abnormalities. In contrast, hypocortisolism appears to be associated with neurodegenerative processes, altered astrocytic metabolism but preserved neuronal density.
TypeArticle
URIhttp://hdl.handle.net/1822/61625
DOI10.1016/j.jpsychires.2007.10.003
ISSN0022-3956
e-ISSN1879-1379
Peer-Reviewedyes
AccessRestricted access (Author)
Appears in Collections:ICVS - Artigos em Revistas Internacionais com Referee

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