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https://hdl.handle.net/1822/61606
Título: | KIT activation in uterine cervix adenosquamous carcinomas by KIT/SCF autocrine/paracrine stimulation loops |
Autor(es): | Martinho, Olga Gonçalves, Alberto Moreira, Marise A. R. Ribeiro, Luiz F. J. Queiroz, Geraldo S. Schmitt, Fernando C. Reis, R. M. Longatto, Adhemar |
Palavras-chave: | Adult Aged Carcinoma, Adenosquamous Enzyme Activation Female Gene Amplification Humans Immunohistochemistry Middle Aged Phosphorylation Polymerase Chain Reaction Polymorphism, Single-Stranded Conformational Proto-Oncogene Proteins c-kit Stem Cell Factor Uterine Cervical Neoplasms Young Adult KIT Mutations Amplification Activation Cervix adenosquamous carcinoma SCF |
Data: | Nov-2008 |
Editora: | Elsevier |
Revista: | Gynecologic Oncology |
Resumo(s): | Objectives. Uterine adenosquamous carcinoma (ASC) is an uncommon, yet, one of the most aggressive cervical cancer subtype. The successful treatment of some tumors, such as gastrointestinal stromal tumors (GISTs), by anti-KIT inhibitors fosters the study of this receptor tyrosine kinase in other malignancies. In the present study, we intended to molecularly characterize KIT in ASC. Methods. In a series of 30 cases, we studied KIT (CD117), KIT phosphorylated/activated form, as well as KIT ligand, stem cell factor (SCF), by immunohistochemistry. We further screened for KIT hotspot mutations (exon 9, 11, 13 and 17) by PCR-SSCP and for KIT gene amplification by Quantitative real-time PCR in CD117 positive cases. Results. We observed CD117 expression in ∼13% of cases, with ∼7% co-expressing SCF, which resulted in KIT phosphorylation/activation. No KIT activating mutations or gene amplification were found, despite the presence of 4q aneuploidy in one case. Conclusions. This is the first study assessing KIT activation and molecular alterations in a large series of rare ASC. Our findings showed the absence of KIT molecular alterations and suggested the presence of KIT activation in a small proportion of cases through KIT/SCF co-expression. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/61606 |
DOI: | 10.1016/j.ygyno.2008.07.011 |
ISSN: | 0090-8258 |
e-ISSN: | 1095-6859 |
Arbitragem científica: | yes |
Acesso: | Acesso restrito autor |
Aparece nas coleções: | ICVS - Artigos em revistas internacionais / Papers in international journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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martinho2008.pdf Acesso restrito! | 649,5 kB | Adobe PDF | Ver/Abrir |