Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/61288

TitleSynergistic action of phage and antibiotics: parameters to enhance the killing efficacy against mono and dual-species biofilms
Author(s)Akturk, Ergun
Oliveira, Hugo
Santos, Sílvio B.
Costa, Susana P.
Kuyumcu, Suleyman
Melo, Luís Daniel Rodrigues
Azeredo, Joana
KeywordsPseudomonas aeruginosa
Staphylococcus aureus
bacteriophage
dual-species
biofilms
antibiotic
synergy
simultaneous
sequential
Issue date25-Jul-2019
PublisherMDPI AG
JournalAntibiotics
CitationAkturk, Ergun; Oliveira, Hugo; Santos, Sílvio B.; Costa, Susana P.; Kuyumcu, Suleyman; Melo, Luís D. R.; Azeredo, Joana, Synergistic action of phage and antibiotics: parameters to enhance the killing efficacy against mono and dual-species biofilms. Antibiotics, 8(3), 103, 2019
Abstract(s)Pseudomonas aeruginosa and Staphylococcus aureus are opportunistic pathogens and are commonly found in polymicrobial biofilm-associated diseases, namely chronic wounds. Their co-existence in a biofilm contributes to an increased tolerance of the biofilm to antibiotics. Combined treatments of bacteriophages and antibiotics have shown a promising antibiofilm activity, due to the profound differences in their mechanisms of action. In this study, 48 h old mono and dual-species biofilms were treated with a newly isolated P. aeruginosa infecting phage (EPA1) and seven different antibiotics (gentamicin, kanamycin, tetracycline, chloramphenicol, erythromycin, ciprofloxacin, and meropenem), alone and in simultaneous or sequential combinations. The therapeutic efficacy of the tested antimicrobials was determined. Phage or antibiotics alone had a modest effect in reducing biofilm bacteria. However, when applied simultaneously, a profound improvement in the killing effect was observed. Moreover, an impressive biofilm reduction (below the detection limit) was observed when gentamicin or ciprofloxacin were added sequentially after 6 h of phage treatment. The effect observed does not depend on the type of antibiotic but is influenced by its concentration. Moreover, in dual-species biofilms it was necessary to increase gentamicin concentration to obtain a similar killing effect as occurs in mono-species. Overall, combining phages with antibiotics can be synergistic in reducing the bacterial density in biofilms. However, the concentration of antibiotic and the time of antibiotic application are essential factors that need to be considered in the combined treatments.
TypeArticle
URIhttp://hdl.handle.net/1822/61288
DOI10.3390/antibiotics8030103
ISSN2079-6382
e-ISSN2079-6382
Publisher versionhttps://www.mdpi.com/journal/antibiotics
Peer-Reviewedyes
AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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