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https://hdl.handle.net/1822/60971
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Campo DC | Valor | Idioma |
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dc.contributor.author | Leal, Tânia | por |
dc.contributor.author | Abrunhosa, Luís | por |
dc.contributor.author | Domingues, Lucília | por |
dc.contributor.author | Venâncio, Armando | por |
dc.contributor.author | Oliveira, Carla | por |
dc.date.accessioned | 2019-07-22T11:45:57Z | - |
dc.date.available | 2019-07-22T11:45:57Z | - |
dc.date.issued | 2019-12 | - |
dc.identifier.citation | Leal, Tânia; Abrunhosa, Luís; Domingues, Lucília; Venâncio, Armando; Oliveira, Carla, BSA-based sample clean-up columns for ochratoxin A determination in wine: method development and validation. Food Chemistry, 300(125204), 2019 | por |
dc.identifier.issn | 0308-8146 | por |
dc.identifier.uri | https://hdl.handle.net/1822/60971 | - |
dc.description.abstract | Analytical chromatographic techniques for mycotoxins control are well established, but they often depend on costly immunoaffinity sample clean-up. Serum albumins, particularly that from bovine origin (BSA), have stable binding affinity towards some mycotoxins, and can be cheaper alternative receptors for sample clean-up due to their wide availability. Thus, this work used BSA immobilized in agarose beads as a novel solid-phase extraction method for quantification of ochratoxin A (OTA) in wine. Constructed BSA-agarose columns could extract OTA efficiently from red wine after its dilution (4-fold) in 0.1 M Tris pH 8.0. The method was linear (R2=0.9999) in the OTA concentration range studied (0.05 to 3.0 g L-1), with recovery rates above 98%. It also showed low detection (0.017 g L-1) and quantification (0.051 g L-1) limits. The efficacy of the BSA-based method was further validated by direct comparison with commercial immunoaffinity columns. Portuguese wines analyzed by both methods had agreeing results. | por |
dc.description.sponsorship | This study was supported by the Portuguese Foundation for Scienceand Technology (FCT) under the scope of the strategic funding of UID/BIO/04469 unit and COMPETE 2020 (POCI-01-0145-FEDER-006684)and BioTecNorte operation (NORTE-01-0145-FEDER-000004) funded by the European Regional Development Fund under the scope of Norte2020 - Programa Operacional Regional do Norte. Luís Abrunhosawas supported by grant UMINHO/BPD/51/2015 from project UID/BIO/04469/2013 financed by FCT/MEC (OE). Carla Oliveira was sup-ported by the FCT grant SFRH/BPD/110640/2015. | por |
dc.language.iso | eng | por |
dc.publisher | Elsevier | por |
dc.relation | UMINHO/BPD/51/2015 | por |
dc.relation | info:eu-repo/grantAgreement/FCT/5876/147337/PT | por |
dc.relation | SFRH/BPD/110640/2015 | por |
dc.rights | openAccess | por |
dc.subject | Ochratoxin A | por |
dc.subject | bovine serum albumin | por |
dc.subject | solid-phase extraction | por |
dc.subject | wine analysis | por |
dc.subject | method validation | por |
dc.title | BSA-based sample clean-up columns for ochratoxin A determination in wine: method development and validation | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | http://www.elsevier.com/locate/issn/03088146 | por |
dc.comments | CEB51852 | por |
oaire.citationVolume | 300 | por |
dc.date.updated | 2019-07-22T10:55:34Z | - |
dc.identifier.doi | 10.1016/j.foodchem.2019.125204 | por |
dc.identifier.pmid | 31336275 | por |
dc.description.publicationversion | info:eu-repo/semantics/publishedVersion | - |
dc.subject.wos | Science & Technology | por |
sdum.journal | Food Chemistry | por |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_51852_1.pdf | 440,98 kB | Adobe PDF | Ver/Abrir |