Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/60971

TitleBSA-based sample clean-up columns for ochratoxin A determination in wine: method development and validation
Author(s)Leal, Tânia
Abrunhosa, Luís
Domingues, Lucília
Venâncio, Armando
Oliveira, Carla
KeywordsOchratoxin A
bovine serum albumin
solid-phase extraction
wine analysis
method validation
Issue dateDec-2019
PublisherElsevier
JournalFood Chemistry
CitationLeal, Tânia; Abrunhosa, Luís; Domingues, Lucília; Venâncio, Armando; Oliveira, Carla, BSA-based sample clean-up columns for ochratoxin A determination in wine: method development and validation. Food Chemistry, 300(125204), 2019
Abstract(s)Analytical chromatographic techniques for mycotoxins control are well established, but they often depend on costly immunoaffinity sample clean-up. Serum albumins, particularly that from bovine origin (BSA), have stable binding affinity towards some mycotoxins, and can be cheaper alternative receptors for sample clean-up due to their wide availability. Thus, this work used BSA immobilized in agarose beads as a novel solid-phase extraction method for quantification of ochratoxin A (OTA) in wine. Constructed BSA-agarose columns could extract OTA efficiently from red wine after its dilution (4-fold) in 0.1 M Tris pH 8.0. The method was linear (R2=0.9999) in the OTA concentration range studied (0.05 to 3.0 g L-1), with recovery rates above 98%. It also showed low detection (0.017 g L-1) and quantification (0.051 g L-1) limits. The efficacy of the BSA-based method was further validated by direct comparison with commercial immunoaffinity columns. Portuguese wines analyzed by both methods had agreeing results.
TypeArticle
URIhttp://hdl.handle.net/1822/60971
DOI10.1016/j.foodchem.2019.125204
ISSN0308-8146
Publisher versionhttp://www.elsevier.com/locate/issn/03088146
Peer-Reviewedyes
AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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