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|Title:||Structure, rheology, and copper-complexation of a hyaluronan-like exopolysaccharide from Vibrio|
Ferreira, A. S.
Coimbra, M. A.
Reis, R. L.
|Citation:||Martin-Pastor M., Ferreira A. S., Moppert X., Nunes C., Coimbra M. A., Reis R. L., Guezennec J., Novoa-Carvallal R. Structure, rheology, and copper-complexation of a hyaluronan-like exopolysaccharide from Vibrio, Carbohydrate Polymers, Vol. 222, pp. 1149992, doi:10.1016/j.carbpol.2019.114999, 2019|
|Abstract(s):||MO245 exopolysaccharide (EPS) was produced in laboratory conditions fromÂ VibrioÂ genus microorganism isolated from bacterial mats found in Moorea Island. Its structure consists of a linear tetrasaccharide repeating unit â 4)-Î²-D-GlcpA-(1â 4)-Î±-D-GalpNAc-(1â 3)-Î²-D-GlcpNAc-(1â 4)-Î²-D-GlcpA-(1â containing covalently-linked 5% of glucose, galactose, and rhamnose, determined by methylation analyses and NMR spectroscopy. The molecular weight, radius of gyration (Rg) and intrinsic viscosity, [Î·], determined by gel permeation chromatography with light scattering and viscosity detection, were 513â Â±â 4â kDa (PDI, 1.42â Â±â 0.01), 6.7â Â±â 0.3â dl/g and 56â Â±â 0.3â nm respectively. The chelation of the EPS with copper divalent ions leads to the instantaneous formation of gels. The structural similitude proposed, based in an equal ratio of GlcA to N-acetylated sugars and in the same type of glyosidic linkages present in the repeating unit (alternated 1â 3 and 1â 4 linkages), is translated into analogous physicochemical properties: MO245 EPS is a flexible polyelectrolyte, with scaling exponents similar to that described for HA. This similitude opens opportunities in future drug delivery, tissue engineering, and cosmetic applications.|
|Access:||Restricted access (UMinho)|
|Appears in Collections:||3B’s - Artigos em revistas/Papers in scientific journals|
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