Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/60886

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dc.contributor.authorVázquez, José A.por
dc.contributor.authorFraguas, J.por
dc.contributor.authorNovoa-Carballal, Ramonpor
dc.contributor.authorReis, R. L.por
dc.contributor.authorPérez-Martín, Ricardo I.por
dc.contributor.authorValcarcel, J.por
dc.date.accessioned2019-07-16T08:42:17Z-
dc.date.issued2019-04-
dc.date.submitted2018-11-
dc.identifier.citationJosé V. A., Fraguas J., Novoa-Carvallal R., Reis R. L., Ricardo P. M. I., Valcarcel J. Optimal isolation and characterisation of chondroitin sulfate from Rabbit fish (Chimaera monstrosa), Carbohydrate Polymers, Vol. 210, pp. 302-313, doi:10.1016/j.carbpol.2019.01.075, 2019por
dc.identifier.issn0144-8617por
dc.identifier.urihttp://hdl.handle.net/1822/60886-
dc.description.abstractChondroitin sulfate (CS) is a glycosaminoglycan widely explored for cartilage regeneration. Its bioactivity is influenced by sulfation degree and pattern, and distinct sulfation in marine CS may open new therapeutic possibilities. In this context, we studied for the first time the isolation and characterisation of CS from Rabbit Fish (Chimaera monstrosa). We propose an efficient process starting with enzymatic hydrolysis, followed by chemical treatments and ending in membrane purification. All steps were optimised by response surface methodology. Chemical treatment by alkaline-hydroalcoholic precipitation led to 99% purity CS suitable for biomedical and pharmaceutical applications, and treatment by alkaline hydrolysis yielded CS adequate for nutraceutical formulations (89% purity). Molecular weight and sulfation profiles were similar for both materials. Gel permeation chromatography analyses resulted in molecular weights (Mn) of 51â 55â kDa. NMR and SAX-HPLC revealed dominant 6S-GalNAc sulfation (4S/6S ratio of 0.4), 17% of GlcA 2S-GalNAc 6S and minor quantities of other disaccharides.por
dc.description.sponsorshipFinanced by projects IBEROS (0245_IBEROS_1_E, POCTEP 2015); CVMar  +  I (0302_CVMAR_I_1_P, POCTEP 2015); BLUEHUMAN (EAPA_151/2016, UE-INTERREG Atlantic Area Programme); and the Portuguese Foundation for Science and Technology (IF/00373/2014 and PTDC/QUI-POL/28117/2017)por
dc.language.isoengpor
dc.publisherElsevierpor
dc.rightsrestrictedAccesspor
dc.subjectCharacterisationpor
dc.subjectChondroitin sulfatepor
dc.subjectC. monstrosa cartilagepor
dc.subjectSulfation patternpor
dc.subjectMolecular weightpor
dc.subjectProcess optimisationpor
dc.subjectResponse surface methodologypor
dc.subjectChondroitin sulfate isolationpor
dc.titleOptimal isolation and characterisation of chondroitin sulfate from Rabbit fish (Chimaera monstrosa)por
dc.typearticle-
dc.peerreviewedyespor
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0144861719300888por
dc.commentshttp://3bs.uminho.pt/node/19887por
oaire.citationStartPage302por
oaire.citationEndPage313por
oaire.citationVolume210por
dc.date.updated2019-07-15T15:24:25Z-
dc.identifier.essn1879-1344por
dc.identifier.doi10.1016/j.carbpol.2019.01.075por
dc.date.embargo10000-01-01-
dc.identifier.pmid30732766por
dc.subject.fosEngenharia e Tecnologia::Biotecnologia Industrialpor
dc.subject.wosScience & Technologypor
sdum.journalCarbohydrate Polymerspor
Appears in Collections:3B’s - Artigos em revistas/Papers in scientific journals

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