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TitleIn vitro adsorption of aflatoxin B1, ochratoxin A, and zearalenone by micronized grape stems and olive pomace in buffer solutions
Author(s)Fernandes, J. -M.
Calado, Thalita
Guimarães, Ana C.
Rodrigues, Miguel A. M.
Abrunhosa, Luís
KeywordsAflatoxin B1
Feed additives
Ochratoxin A
Issue dateAug-2019
JournalMycotoxin Research
CitationFernandes, J. -M.; Calado, Thalita; Guimarães, Ana C.; Rodrigues, Miguel A. M.; Abrunhosa, Luís, In vitro adsorption of aflatoxin B1, ochratoxin A, and zearalenone by micronized grape stems and olive pomace in buffer solutions. Mycotoxin Research, 35(3), 243-252, 2019
Abstract(s)This work characterizes the adsorption of aflatoxin B1 (AFB1), ochratoxin A (OTA), and zearalenone (ZEN) by dry micronized olive pomace (OliPom) and grape stems (GrapStem). Their performance was compared with that of three other materials, activated carbon (ActCarb), bentonite (Bent), and a commercial product (ComProd). Experiments were conducted in vitro at several pH values using buffer solutions. For OTA and ZEA, the strongest adsorbent was ActCarb, with 5 mg/mL being sufficient to bind >99% of all the mycotoxins. For AFB1, ComProd and Bent were the most effective adsorbents, as 0.5 mg/mL bound >95% of this mycotoxin. Among the two agro by-products, GrapStem was the strongest binder, with 10 mg/mL being sufficient to bind at least 90% of all the mycotoxins (except OTA at pH 7). OliPom was the least efficient material, but at a concentration of 30 mg/mL, its performance was similar to GrapStem. Adsorption isotherms were calculated, and ActCarb showed the maximum adsorption capacity (Qmax), with values that ranged from 19 to 24 g/mg for pH 2 and from 17 to 20 g/mg for pH 7. ComProd, Bent, and GrapStem showed more similar Qmax between them (1.44.4 g/mg for pH 2 and 0.54.8 g/mg for pH 7).
Publisher version
AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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