Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/60440

TitleQuantification of drugs encapsulated in liposomes by 1H NMR
Author(s)Guimarães, Diana Isabel Pereira
Noro, Jennifer Martins
Loureiro, Ana Isabel Sá
Cavaco-Paulo, Artur
Nogueira, Eugénia Sofia Costa
KeywordsLiposomes
Drugs
QuantificationH NMR
UV-Vis
HPLC-UV/Vis
1 H NMR
Quantification
H-1 NMR
Issue dateJul-2019
PublisherElsevier
JournalColloids and Surfaces B: Biointerfaces
CitationGuimarães, D., Noro, J., Loureiro, A., Cavaco-Paulo, A., & Nogueira, E. (2019). Quantification of drugs encapsulated in liposomes by 1H NMR. Colloids and Surfaces B: Biointerfaces, 179, 414-420
Abstract(s)Liposomes are one of the most important and extensively studied drug delivery system due to their ability to encapsulate different kinds of drugs. Exploiting the advantages of 1H Nuclear Magnetic Resonance (NMR) spectrometry, we established a rapid and easy method for quantification of drugs encapsulated in liposomes. An internal standard, pyridine, was used for quantitative determination of drug concentration. Two different drugs were involved in this work, one hydrophilic, methotrexate disodium salt, and another hydrophobic, tamoxifen. The specificity and selectivity of the suggested method were evaluated by the absence of overlapping of at least one signal of each drug with pyridine in the NMR spectrum. The accuracy and precision of the method were assessed by adding a known amount of each drug to unloaded liposomes. Results obtained by quantitative NMR (qNMR) were validated and confirmed by comparing with two other traditional techniques, Ultraviolet - Visible (UV-Vis) spectrophotometry and High-Performance Liquid Chromatography (HPLC). It was found that the results were consistent with the ones obtained from our proposed qNMR method. Considering all the experiments conducted in this study, we deliberate that qNMR can be a suitable tool for the determination of drugs encapsulated in liposomes.
TypeArticle
URIhttp://hdl.handle.net/1822/60440
DOI10.1016/j.colsurfb.2019.03.039
ISSN0927-7765
e-ISSN0927-7765
Publisher versionhttp://www.journals.elsevier.com/colloids-and-surfaces-b-biointerfaces/
Peer-Reviewedyes
AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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