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dc.contributor.authorCastro, Joanapor
dc.contributor.authorMachado, Danielapor
dc.contributor.authorCerca, Nunopor
dc.identifier.citationCastro, Joana; Machado, Daniela; Cerca, Nuno, Unveiling the role of Gardnerella vaginalis in polymicrobial Bacterial Vaginosis (BV) biofilms: the impact of other vaginal pathogens living as neighbors. ISME Journal, 13, 1306-1317, 2018por
dc.description.abstractBacterial vaginosis (BV) is characterized by a highly structured polymicrobial biofilm, which is strongly adhered to the vaginal epithelium and primarily consists of the bacterium Gardnerella vaginalis. However, despite the presence of other BV-associated bacteria, little is known regarding the impact of other species on BV development. To gain insight into BV progress, we analyzed the ecological interactions between G. vaginalis and 15 BV-associated microorganisms using a dual-species biofilm model. Bacterial populations were quantified using a validated peptide nucleic acid fluorescence in situ hybridization approach. Furthermore, biofilm structure was analyzed by confocal laser scanning microscopy. In addition, bacterial coaggregation ability was determined as well as the expression of key virulence genes. Remarkably, our results revealed distinct biofilm structures between each bacterial consortium, leading to at least three unique dual-species biofilm morphotypes. Furthermore, our transcriptomic findings seem to indicate that Enterococcus faecalis and Actinomyces neuii had a higher impact on the enhancement of G. vaginalis virulence, while the other tested species had a lower or no impact on G. vaginalis virulence. This study casts a new light on how BV-associated species can modulate the virulence aspects of G. vaginalis, contributing to a better understanding of the development of BV-associated biofilms.por
dc.description.sponsorshipThis study was supported by the PortugueseFoundation for Science and Technology (FCT) by the fundedproject PTDC/BIA-MIC/28271/2017, under the scope of COMPETE2020 (POCI-01-0145-FEDER-028271) and by the strategic fundingof unit UID/BIO/04469/2013. The funders had no role in studydesign, data collection and analysis, decision to publish, or preparationof the manuscript. JC had an individual FCT fellowship (SFRH/BD/93963/2013). NC is an Investigador FCT. This manuscript wasedited by American Manuscript Editors (
dc.publisherSpringer Naturepor
dc.titleUnveiling the role of Gardnerella vaginalis in polymicrobial Bacterial Vaginosis (BV) biofilms: the impact of other vaginal pathogens living as neighborspor
oaire.citationConferencePlaceUnited Kingdom-
dc.subject.wosScience & Technologypor
sdum.journalISME Journalpor
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