Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/59843

TitleIn silico approach for unveiling the glycoside hydrolase activities in Faecalibacterium prausnitzii through a systematic and integrative large-scale analysis
Author(s)Blanco, Guillermo
Sánchez, Borja
Fdez-Riverola, Florentino
Margolles, Abelardo
Lourenço, Anália
Keywordsfermentable sugars
glycoside hydrolases
bioactivity
Faecalibacterium prausnitzii
computational screening
Issue date2019
PublisherFrontiers Media
JournalFrontiers in Microbiology
CitationBlanco, Guillermo; Sánchez, Borja; Fdez-Riverola, Florentino; Margolles, Abelardo; Lourenço, Anália, In silico approach for unveiling the glycoside hydrolase activities in Faecalibacterium prausnitzii through a systematic and integrative large-scale analysis. Frontiers in Microbiology, 10(517), 2019
Abstract(s)This work presents a novel in silico approach to the prediction and characterisation of the glycolytic capacities of the beneficial intestinal bacterium Faecalibacterium prausnitzii. Available F. prausnitzii genomes were explored taking the glycolytic capacities of F. prausnitzii SL3/3 and F. prausnitzii L2-6 as reference. The comparison of the generated glycolytic profiles offered insights into the particular capabilities of F. prausnitzii SL3/3 and F. prausnitzii L2-6 as well as the potential of the rest of strains. Glycoside hydrolases were mostly detected in the pathways responsible for the starch and sucrose metabolism and the biosynthesis of secondary metabolites, but this analysis also identified some other potentially interesting, but still uncharacterised activities, such as several hexosyltransferases and some hydrolases. Gene neighbourhood maps offered additional understanding of the genes coding for relevant glycoside hydrolases. Although information about the carbohydrate preferences of F. prausnitzii is scarce, the in silico metabolic predictions were consistent with previous knowledge about the impact of fermentable sugars on the growth promotion and metabolism of F. prausnitzii. So, while the predictions still need to be validated using culturing methods, the approach holds the potential to be reproduced and scaled to accommodate the analysis of other strains (or even families and genus) as well as other metabolic activities. This will allow the exploration of novel methodologies to design or obtain targeted probiotics for F. prausnitzii and other strains of interest.
TypeArticle
URIhttp://hdl.handle.net/1822/59843
DOI10.3389/fmicb.2019.00517
ISSN1664-302X
e-ISSN1664-302X
Publisher versionhttp://journal.frontiersin.org/journal/microbiology
Peer-Reviewedyes
AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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