Please use this identifier to cite or link to this item:

Full metadata record
DC FieldValueLanguage
dc.contributor.authorReis-Filho, Jorge S.por
dc.contributor.authorSimpson, Pete T.por
dc.contributor.authorFulford, Laura G.por
dc.contributor.authorMartins, Albinopor
dc.contributor.authorSchmitt, Fernando C.por
dc.description.abstractDeltaN-p63 isoforms may act as oncogenes owing to their ability to bind to p53-reporter genes without inciting their transcription, thus blocking the p53-driven cell cycle arrest and apoptosis. A novel mechanism linking p63 and Wnt pathways has recently been proposed. Briefly, in vitro studies using squamous cell carcinoma cell lines have suggested that DeltaN-p63 may block the phosphorylation of beta-catenin, leading to its nuclear accumulation and triggering beta-catenin-responsive transcription of genes related to proliferation and oncogenic biological behavior. To test this new mechanism, the coexpression of DeltaN-p63 and beta-catenin was evaluated in a large cohort of human neoplasms.Two serial sections of TARP-4 multi-tumor tissue microarray, composed of 51 normal tissue cores and 400 human neoplasms [breast (n = 75), colon (n = 75), lung (n = 75), prostate (n = 75) and ovary (n = 50) neoplasms, melanoma (n = 25), and glioblastoma (n = 25)] were subjected to immunohistochemistry with DeltaN-p63 and beta-catenin monoclonal antibodies. p63 nuclear expression and beta-catenin membranous, cytoplasmic, membranous + cytoplasmic, and nuclear localization were evaluated.DeltaN-p63 expression and beta-catenin nuclear localization were found in 92.6% and 0% of squamous cell carcinomas, 8.9% and 0% of breast carcinomas, 13.8% and 0% of lung adenocarcinomas, 1.4% and 23.2% of colon adenocarcinomas, 0% and 4.8% of prostate adenocarcinomas, 11.1% and 5% of ovary carcinomas, 9.0% and 9.1% of malignant melanomas, and 12.5% and 40.0% of glioblastomas, respectively. No statistically significant association between DeltaN-p63 and nuclear beta-catenin expression was found for all tumors.At variance with squamous cell carcinoma cell lines, p63-driven nuclear accumulation of beta-catenin is an unusual phenomenon in human neoplasms. Caution should be exercised when translating the results of studies performed on cell lines to human neoplasms.por
dc.description.sponsorshipJS Reis-Filho is the recipient of the Gordon Signy International Fellowship Award and is partially supported by a PhD grant (Ref.: SFRH/BD/5386/2001) from the Fundação para a Ciência e a Tecnologia (FCT), Portugal and Programa Operacional Ciência, Tecnologia e Inovação (POCTI), Ref. POCTI/CBO/45157/2002.por
dc.publisherUrban & Fischer Verlagpor
dc.titlep63-driven nuclear accumulation of β-Catenin is not a frequent event in human neoplasmspor
dc.subject.wosScience & Technologypor
sdum.journalPathology Research and Practicepor
Appears in Collections:ICVS - Artigos em Revistas Internacionais com Referee

Files in This Item:
File Description SizeFormat 
  Restricted access
115,63 kBAdobe PDFView/Open

Partilhe no FacebookPartilhe no TwitterPartilhe no DeliciousPartilhe no LinkedInPartilhe no DiggAdicionar ao Google BookmarksPartilhe no MySpacePartilhe no Orkut
Exporte no formato BibTex mendeley Exporte no formato Endnote Adicione ao seu ORCID