Utilize este identificador para referenciar este registo: https://hdl.handle.net/1822/58885

TítuloAntimicrobial assessment of phage therapy using a porcine model of biofilm infection
Autor(es)Milho, Catarina
Andrade, M.
Boas, Diana Patrícia Andrade Vilas
Alves, Diana
Sillankorva, Sanna
Palavras-chaveChronic wounds
Porcine skin model
Biofilms
Bacteriophages
Data25-Fev-2019
EditoraElsevier
RevistaInternational Journal of Applied Pharmaceutics
CitaçãoMilho, C.; Andrade, M.; Vilas Boas, Diana; Alves, Diana; Sillankorva, Sanna, Antimicrobial assessment of phage therapy using a porcine model of biofilm infection. International Journal of Pharmaceutics, 557, 112-123, 2019
Resumo(s)Antibiotic resistant bacterial communities persist in many types of wounds, chronic wounds in particular, in the form of biofilms. Biofilm formation is a major cause of severe infections and the main reason for a negative treatment outcome and slow healing progression. Chronic wounds are a silent epidemic essentially affecting people with co-morbid conditions such as diabetes and obesity and elderly persons particularly those with movement limitations. The development of complementary and alternative effective strategies to antibiotics for the treatment of chronic wounds is highly desired. Phage therapy constitutes a very promising approach in the control of topical microbial populations. In this work newly isolated phages were tested for their efficacy to control bacterial species that predominate in chronic wounds. Phage effectiveness was studied on 24-h old biofilms formed in polystyrene microplates and in porcine skin explants using two treatment approaches: individual phage and a cocktail of phages against four main pathogens commonly isolated from chronic wounds. The two models produced variations in the surface colonization ability, assessed by viable bacterial counts and microscopy visualization after using peptide nucleic acid (PNA) or locked nucleic acid probes (LNA) and 2-O-methyl (2-OMe) in fluorescence in situ hybridization (FISH), and in the phage-host interactions. Phages alone and combined caused greater reductions in the number of viable cells when biofilms had been formed on porcine skins and with greater variations detected at 4 h and 24 h of sampling. These results suggest that porcine skin models should be preferentially used to assess the use of phages and phage cocktails intended for topical use in order to understand the fate, throughout treatment time, of the population when dealing with biofilm-related infections.
TipoArtigo
URIhttps://hdl.handle.net/1822/58885
DOI10.1016/j.ijpharm.2018.12.004
ISSN0378-5173
e-ISSN0378-5173
Versão da editorahttp://www.elsevier.com/locate/issn/03785173
Arbitragem científicayes
AcessoAcesso aberto
Aparece nas coleções:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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