Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/58826

TitleThe Adhesion GPCR GPR125 is specifically expressed in the choroid plexus and is upregulated following brain injury
Author(s)Pickering, Chris
Hägglund, Maria
Szmydynger-Chodobska, Joanna
Marques, Fernanda
Palha, Joana Almeida
Waller, Linn
Chodobski, Adam
Fredriksson, Robert
Lagerström, Malin C.
Schiöth, Helgi B.
KeywordsAnimals
Base Sequence
Brain Injuries
Choroid Plexus
Gene Expression Profiling
Hippocampus
Humans
Immunohistochemistry
In Situ Hybridization
Inflammation
Keratins
Lipopolysaccharides
Male
Membrane Glycoproteins
Membrane Proteins
Mice
Platelet Glycoprotein GPIb-IX Complex
RNA, Messenger
Rats
Receptors, G-Protein-Coupled
Time Factors
Up-Regulation
Issue date2008
PublisherBioMed Central
JournalBMC Neuroscience
Abstract(s)Background: GPR125 belongs to the family of Adhesion G protein-coupled receptors (GPCRs). A single copy of GPR125 was found in many vertebrate genomes. We also identified a Drosophila sequence, DmCG15744, which shares a common ancestor with the entire Group III of Adhesion GPCRs, and also contains Ig, LRR and HBD domains which were observed in mammalian GPR125.Results: We found specific expression of GPR125 in cells of the choroid plexus using in situ hybridization and protein-specific antibodies and combined in situ/immunohistochemistry co-localization using cytokeratin, a marker specific for epithelial cells. Induction of inflammation by LPS did not change GPR125 expression. However, GPR125 expression was transiently increased ( almost 2-fold) at 4 h after traumatic brain injury (TBI) followed by a decrease ( approximately 4-fold) from 2 days onwards in the choroid plexus as well as increased expression ( 2-fold) in the hippocampus that was delayed until 1 day after injury.Conclusion: These findings suggest that GPR125 plays a functional role in choroidal and hippocampal response to injury.
Typearticle
URIhttp://hdl.handle.net/1822/58826
DOI10.1186/1471-2202-9-97
ISSN1471-2202
Peer-Reviewedyes
AccessopenAccess
Appears in Collections:ICVS - Artigos em Revistas Internacionais com Referee

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