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|Title:||The Adhesion GPCR GPR125 is specifically expressed in the choroid plexus and is upregulated following brain injury|
Palha, Joana Almeida
Lagerström, Malin C.
Schiöth, Helgi B.
Gene Expression Profiling
In Situ Hybridization
Platelet Glycoprotein GPIb-IX Complex
|Abstract(s):||Background: GPR125 belongs to the family of Adhesion G protein-coupled receptors (GPCRs). A single copy of GPR125 was found in many vertebrate genomes. We also identified a Drosophila sequence, DmCG15744, which shares a common ancestor with the entire Group III of Adhesion GPCRs, and also contains Ig, LRR and HBD domains which were observed in mammalian GPR125.Results: We found specific expression of GPR125 in cells of the choroid plexus using in situ hybridization and protein-specific antibodies and combined in situ/immunohistochemistry co-localization using cytokeratin, a marker specific for epithelial cells. Induction of inflammation by LPS did not change GPR125 expression. However, GPR125 expression was transiently increased ( almost 2-fold) at 4 h after traumatic brain injury (TBI) followed by a decrease ( approximately 4-fold) from 2 days onwards in the choroid plexus as well as increased expression ( 2-fold) in the hippocampus that was delayed until 1 day after injury.Conclusion: These findings suggest that GPR125 plays a functional role in choroidal and hippocampal response to injury.|
|Appears in Collections:||ICVS - Artigos em Revistas Internacionais com Referee|