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TitleChondrogenesis-inductive nanofibrous substrate using both biological fluids and mesenchymal stem cells from an autologous source
Author(s)Casanova, Marta Alexandra Rodrigues
Alves da Silva, M.
Costa-Pinto, A. R.
Reis, R. L.
Martins, A.
Neves, N. M.
KeywordsChondrogenic differentiation
Electrospun nanofibrous meshes
Insulin-like growth factor-I (IGF-I)
Platelet lysates
Transforming growth factor-β3 (TGF-β3)
Transforming growth factor-beta 3 (TGF-beta 3)
Issue dateMay-2019
JournalMaterials Science and Engineering: C
CitationCasanova M. R., Alves da Silva M., Costa-Pinto A. R., Reis R. L., Martins A., Neves N. M. Chondrogenesis-inductive nanofibrous substrate using both biological fluids and mesenchymal stem cells from an autologous source, Materials Science and Engineering: C, Vol. 98, pp. 1169-1178, doi:10.1016/j.msec.2019.01.069, 2019
Abstract(s)During the last decade, many cartilage tissue engineering strategies have been developed, being the stem cell-based approach one of the most promising. Transforming Growth Factor-β3 (TGF-β3) and Insulin-like Growth Factor-I (IGF-I) are key proteins involved in the regulation of chondrogenic differentiation. Therefore, these two growth factors (GFs) were immobilized at the surface of a single electrospun nanofibrous mesh (NFM) aiming to differentiate human Bone Marrow-derived Mesenchymal Stem Cells (hBM-MSCs). The immobilization of defined antibodies (i.e. anti-TGF-β3 and anti-IGF-I) allows the selective retrieval of the abovementioned GFs from human platelet lysates (PL). Biochemical assays, involving hBM-MSCs cultured on biofunctional nanofibrous substrates under basal culture medium during 28⠯days, confirm the biological activity of bound TGF-β3 and IGF-I. Specifically, the typical spherical morphology of chondrocytes and the immunolocalization of collagen type II confirmed the formation of a cartilaginous ECM. Therefore, the proposed biofunctional nanofibrous substrate is able to promote chondrogenesis.
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Appears in Collections:3B’s - Artigos em revistas/Papers in scientific journals

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