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|Title:||Hyaluronan density influences adhesion, morphology and migration of cancer cells|
Soares da Costa, D.
Reis, R. L.
|Journal:||Abstracts of Papers of the American Chemical Society|
|Citation:||Carvalho A., Soares da Costa D., Reis R. L., Pashkuleva I. Hyaluronan density influences adhesion, morphology and migration of cancer cells, ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, Vol. 256, pp. 486, 0065-7727, 2018|
|Abstract(s):||Hyaluronan (HA) is a linear non-sulfated glycosaminoglycan present in the extracellular matrix and known to modulate cell-cell and cell-ECM interactions. In cancer, the synthesis, degradation and signaling of HA is altered. For instance, its main receptor, CD44, is overexpressed in several types of cancer and has been correlated with disease progression through cancer cell proliferation, migration and chemoresistance. Herein, we investigated the behavior of breast cancer cells with diﬀerent CD44 expression and invasion proﬁle on HA density gradients. These gradients were achieved by deposition of colloidal gold (Au) on amino-functionalized surfaces at diﬀerent ionic strengths and following binding of end-on thiol modiﬁed HA on the Au. At low HA density, small number of adherent round cells were found for all studied cell lines. Cells adherent to the areas with high HA density presented a spindle-like morphology. The diﬀerences were more pronounced for cells overexpressing CD44. These cells also form long ﬁlopodia when adhered on areas with middle and high HA density. Of note, colocalization of CD44 and actin was observed at the ﬁlopodias edges. Cell motility was also aﬀected by the gradient – at low densities cells presented higher motility, which decreased with the increase of HA density. Besides this common trend, we observed diﬀerences among the studied cells. CD44 cells had shorter persistent length displacement than CD44 and CD44cells. Upon CD44 blockage, all types of cells (CD44++, CD44+, and CD44-) behave similarly . These results suggest that cells recognize HA gradients through CD44 receptors and that the HA density can be used to sort cells with diﬀerent expression of this receptor.|
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