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https://hdl.handle.net/1822/58495
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Campo DC | Valor | Idioma |
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dc.contributor.author | Zhang, Yongchun | por |
dc.contributor.author | Yang, Ying | por |
dc.contributor.author | Jiang, Ming | por |
dc.contributor.author | Huang, Sarah Xuelian | por |
dc.contributor.author | Zhang, Wanwei | por |
dc.contributor.author | Al Alam, Denise | por |
dc.contributor.author | Danopoulos, Soula | por |
dc.contributor.author | Mori, Munemasa | por |
dc.contributor.author | Chen, Ya-Wen | por |
dc.contributor.author | Balasubramanian, Revathi | por |
dc.contributor.author | Chuva de Sousa Lopes, Susana M. | por |
dc.contributor.author | Serra, Carlos | por |
dc.contributor.author | Bialecka, Monika | por |
dc.contributor.author | Kim, Eugene | por |
dc.contributor.author | Lin, Sijie | por |
dc.contributor.author | Toste de Carvalho, Ana Luisa Rodrigues | por |
dc.contributor.author | Riccio, Paul N. | por |
dc.contributor.author | Cardoso, Wellington V. | por |
dc.contributor.author | Zhang, Xin | por |
dc.contributor.author | Snoeck, Hans-Willem | por |
dc.contributor.author | Que, Jianwen | por |
dc.date.accessioned | 2019-01-23T14:43:19Z | - |
dc.date.issued | 2018-10-04 | - |
dc.identifier.citation | Zhang, Y., Yang, Y., Jiang, M., et. al. (2018). 3D modeling of esophageal development using human PSC-derived basal progenitors reveals a critical role for Notch signaling. Cell stem cell, 23(4), 516-529 | por |
dc.identifier.issn | 1934-5909 | por |
dc.identifier.uri | https://hdl.handle.net/1822/58495 | - |
dc.description.abstract | Pluripotent stem cells (PSCs) could provide a powerful system to model development of the human esophagus, whose distinct tissue organization compared to rodent esophagus suggests that developmental mechanisms may not be conserved between species. We therefore established an efficient protocol for generating esophageal progenitor cells (EPCs) from human PSCs. We found that inhibition of TGF-ß and BMP signaling is required for sequential specification of EPCs, which can be further purified using cell-surface markers. These EPCs resemble their human fetal counterparts and can recapitulate normal development of esophageal stratified squamous epithelium during in vitro 3D cultures and in vivo. Importantly, combining hPSC differentiation strategies with mouse genetics elucidated a critical role for Notch signaling in the formation of this epithelium. These studies therefore not only provide an efficient approach to generate EPCs, but also offer a model system to study the regulatory mechanisms underlying development of the human esophagus. | por |
dc.description.sponsorship | Work in the Que lab is partly supported by R01DK113144, R01DK100342, and R01HL132996 (J.Q.), and the Price Family Foundation. S.D. acknowledges a fellowship support from the Hastings Center for Pulmonary Research (USC). Flow cytometry was performed in the Columbia Center for Translational Immunology (CCTI) Flow Cytometry Core at Columbia University Medical Center, supported in part by the Office of the Director, NIH under the award S10OD020056. | por |
dc.language.iso | eng | por |
dc.publisher | Elsevier | por |
dc.rights | closedAccess | por |
dc.subject | Human embryonic stem cells | por |
dc.subject | Human-induced pluripotent stem cell | por |
dc.subject | Basal cells | por |
dc.subject | Esophagus | por |
dc.subject | Organoids | por |
dc.subject | BMP | por |
dc.subject | TGF-ß | por |
dc.subject | WNT | por |
dc.subject | NOTCH | por |
dc.title | 3D modeling of esophageal development using human PSC-Derived basal progenitors reveals a critical role for notch signaling | por |
dc.type | article | por |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | https://www.sciencedirect.com/science/article/pii/S1934590918303941 | por |
oaire.citationStartPage | 516 | por |
oaire.citationEndPage | 529.e5 | por |
oaire.citationIssue | 4 | por |
oaire.citationVolume | 23 | por |
dc.identifier.doi | 10.1016/j.stem.2018.08.009 | por |
dc.identifier.pmid | 30244870 | por |
dc.subject.fos | Ciências Médicas::Medicina Básica | por |
dc.description.publicationversion | info:eu-repo/semantics/publishedVersion | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Cell Stem Cell | por |
Aparece nas coleções: | ICVS - Artigos em revistas internacionais / Papers in international journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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zhang2018.pdf Acesso restrito! | 7,58 MB | Adobe PDF | Ver/Abrir |