Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/58405

TitleA Passive Microfluidic Device Based on Crossflow Filtration for Cell Separation Measurements: A Spectrophotometric Characterization
Author(s)Faustino, Vera
Catarino, Susana Oliveira
Pinho, Diana
Lima, Rui Alberto Madeira Macedo
Minas, Graça
KeywordsMicrofluidics
Red blood cells
Separation
Cross-flow filtration
Spectrophotometry
Issue date1-Dec-2018
PublisherMDPI
JournalBiosensors
CitationFaustino, V., Catarino, S., Pinho, D., Lima, R., & Minas, G. (2018). A Passive Microfluidic Device Based on Crossflow Filtration for Cell Separation Measurements: A Spectrophotometric Characterization. Biosensors, 8(4), 125
Abstract(s)Microfluidic devices have been widely used as a valuable research tool for diagnostic applications. Particularly, they have been related to the successful detection of different diseases and conditions by assessing the mechanical properties of red blood cells (RBCs). Detecting deformability changes in the cells and being able to separate those cells may be a key factor in assuring the success of detection of some blood diseases with diagnostic devices. To detect and separate the chemically modified RBCs (mimicking disease-infected RBCs) from healthy RBCs, the present work proposes a microfluidic device comprising a sequence of pillars with different gaps and nine different outlets used to evaluate the efficiency of the device by measuring the optical absorption of the collected samples. This latter measurement technique was tested to distinguish between healthy RBCs and RBCs chemically modified with glutaraldehyde. The present study indicates that it was possible to detect a slight differences between the samples using an optical absorption spectrophotometric setup. Hence, the proposed microfluidic device has the potential to perform in one single step a partial passive separation of RBCs based on their deformability.
TypeArticle
URIhttp://hdl.handle.net/1822/58405
DOI10.3390/bios8040125
ISSN2079-6374
e-ISSN2079-6374
Publisher versionhttps://www.mdpi.com/2079-6374/8/4/125
Peer-Reviewedyes
AccessOpen access
Appears in Collections:DEI - Artigos em revistas internacionais

Files in This Item:
File Description SizeFormat 
biosensors-08-00125 - published.pdf998,88 kBAdobe PDFView/Open

Partilhe no FacebookPartilhe no TwitterPartilhe no DeliciousPartilhe no LinkedInPartilhe no DiggAdicionar ao Google BookmarksPartilhe no MySpacePartilhe no Orkut
Exporte no formato BibTex mendeley Exporte no formato Endnote Adicione ao seu ORCID