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TitleBiomolecular interactions of lysosomotropic surfactants with cytochrome c and its effect on the protein conformation: A biophysical approach
Author(s)Janek, Tomasz
Czelen, Przemyslaw
Gudiña, Eduardo José
Rodrigues, L. R.
Czyznikowska, Zaneta
KeywordsLysosomotropic surfactant
Fluorescence quenching
Circular dichroism
Molecular dynamic simulations
Cytochrome c
Issue dateApr-2019
JournalInternational Journal of Biological Macromolecules
CitationJanek, Tomasz; Czelen, Przemyslaw; Gudiña, Eduardo J.; Rodrigues, Lígia R.; Czyznikowska, Zaneta, Biomolecular interactions of lysosomotropic surfactants with cytochrome c and its effect on the protein conformation: A biophysical approach. International Journal of Biological Macromolecules, 126, 1177-1185, 2019
Abstract(s)The molecular interactions between two single-chain lysosomotropic surfactants DMM-11 (2-Dodecanoyloxyethyl)trimethylammonium bromide) and DMPM-11 (2-Dodecanoyloxypropyl)trimethylammonium bromide) with a small heme-protein (cytochrome c (cyt-c)) in Hepes buffer (pH = 7.4) were extensively investigated by surface tension, dynamic light scattering (DLS), circular dichroism (CD) and fluorescence spectroscopy in combination with molecular dynamic simulation techniques. The results demonstrated that surfactants can destroy the hydrophobic cavity of cyt-c, make the α-helical become loose and convert it into the β-sheet structure. The interactions between surfactants and cyt-c are mainly hydrophobic. Molecular modelling approaches were also used to gather a deeper insight on the binding of lysosomotropic surfactants with cyt-c and the in silico results were found to be in good agreement with the experimental ones. This study provides a molecular basis for the applications of protein-surfactant complexes in biological, food, pharmaceutical, industrial and cosmetic systems
Publisher version
AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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