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TitleBiotechnological production of xylitol: engineering industrial Saccharomyces cerevisiae for valorization of lignocellulosic biomass
Author(s)Sara Baptista
Romaní, Aloia
Cunha, Joana T.
Domingues, Lucília
Industrial Saccharomyces cerevisiae
Fed-batch fermentation
Issue date24-Oct-2018
CitationSara Baptista; Romaní, Aloia; Cunha, Joana T.; Domingues, Lucília, Biotechnological production of xylitol: engineering industrial Saccharomyces cerevisiae for valorization of lignocellulosic biomass. 4-CIAB - Proceedings of the 4th Iberoamerican Congress on Biorefineries. Vol. Actas 48, Jaén, Spain, 24-26 October, 481-481, 2018. ISBN: 978-84-9159-144-3
Abstract(s)The use of renewable biomass, such as lignocellulosic materials, for the production of biofuels and chemicals within a biorefinery scheme contributes to achieve a sustainable development. Xylitol has been identified as one of the top 12 value added chemicals to be obtained from biomass, and can be produced from hemicellulose-derived xylose through biotechnological processes [1]. In this work, xylitol was produced from xylose (using glucose as co-substrate for co-factor regeneration) in batch fermentations by the industrial Saccharomyces cerevisiae PE-2 strain (over)expressing (1) a wild type xylose reductase from Pichia spititis (XR); (2) a NADH-preferable xylose reductase mutant (mut-XR) from Pichia spititis and (3) the endogenous GRE3 gene which encodes for an unspecific aldose reductase (AR). Maximum yield (0.98 g g-1) was obtained by the strain overexpressing the GRE3 gene. Moreover, the recombinant strain PE-2-GRE3 showed significantly higher xylitol productivity than the laboratory strain, CENPK.113-5D overexpressing the same gene. This strain (PE-2-GRE3) was selected for bioconversion of 160 g L-1 of xylose in a fed-batch fermentation, which resulted in 149 g L-1 of xylitol concentration with a productivity of 1.2 g L-1 h-1. These results open new perspectives and opportunities for the valorisation of hemicellulosic hydrolysates through the production of xylitol within a biorefinery concept.
TypePanel presentation
Publisher version
AccessOpen access
Appears in Collections:CEB - Resumos em Livros de Atas / Abstracts in Proceedings

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