Utilize este identificador para referenciar este registo: http://hdl.handle.net/1822/58272

TítuloA simple architecture with self-assembled monolayers to build immunosensors for detecting the pancreatic cancer biomarker CA19-9
Autor(es)Soares, Andrey Coatrini
Soares, Juliana Coatrini
Shimizu, Flavio Makoto
Rodrigues, Valquiria da Cruz
Awan, Iram Taj
Melendez, Matias Eliseo
Piazzetta, Maria Helena Oliveira
Gobbi, Angelo Luiz
Reis, R. M.
Fregnani, José Humberto T. G.
Carvalho, André Lopes
Oliveira, Osvaldo N.
EditoraRoyal Soc Chemistry
CitaçãoSoares, A. C., Soares, J. C., Shimizu, F. M., et. al. (2018). A simple architecture with self-assembled monolayers to build immunosensors for detecting the pancreatic cancer biomarker CA19-9. Analyst
Resumo(s)The challenge of the early diagnosis of pancreatic cancer in routine clinical practice requires low-cost means of detection, and this may be achieved with immunosensors based on electrical or electrochemical principles. In this paper, we report a potentially low-cost immunosensor built with interdigitated gold electrodes coated with a self-assembled monolayer and a layer of anti-CA19-9 antibodies, which is capable of detecting the pancreatic cancer biomarker CA19-9 using electrical impedance spectroscopy. Due to specific, irreversible adsorption of CA19-9 onto its corresponding antibody, according to data from polarization-modulated infrared reflection absorption spectroscopy (PM-IRRAS), the immunosensor is highly sensitive and selective. It could detect CA19-9 in commercial samples with a limit of detection of 0.68 U mL−1, in addition to distinguishing between blood serum samples from patients with different concentrations of CA19-9. Furthermore, by treating the capacitance data with information visualization methods, we were able to verify the selectivity and robustness of the immunosensor with regard to false positives, as the samples containing higher CA19-9 concentrations, including those from tumor cells, could be distinguished from those with possible interferents.
DescriçãoAccepted Manuscript
Versão da editorahttps://pubs.rsc.org/en/content/articlehtml/2018/an/c8an00430g
Arbitragem científicayes
AcessoembargoedAccess (1 Year)
Aparece nas coleções:ICVS - Artigos em Revistas Internacionais com Referee

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