Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/58045

TitleThe Dynamics of Interleukin-10-Afforded Protection during Dextran Sulfate Sodium-Induced Colitis
Author(s)Cardoso, Ana
Castro, António G.
Martins, Ana Catarina
Carriche, Guilhermina M.
Murigneux, Valentine
Castro, Isabel
Cumano, Ana
Vieira, Paulo
Saraiva, Margarida
KeywordsInterleukin-10
Macrophages
Inflammation
Colitis
Therapy
Issue date2018
PublisherFrontiers Media
JournalFrontiers in Immunology
CitationCardoso, A., Gil Castro, A., Martins, A. C., et. al.(2018). The Dynamics of interleukin-10-afforded Protection during Dextran sulfate sodium-induced colitis. Frontiers in immunology, 9, 400
Abstract(s)Inflammatory bowel disease encompasses a group of chronic-inflammatory conditions of the colon and small intestine. These conditions are characterized by exacerbated inflammation of the organ that greatly affects the quality of life of patients. Molecular mechanisms counteracting this hyperinflammatory status of the gut offer strategies for therapeutic intervention. Among these regulatory molecules is the anti-inflammatory cytokine interleukin (IL)-10, as shown in mice and humans. Indeed, IL-10 signaling, particularly in macrophages, is essential for intestinal homeostasis. We sought to investigate the temporal profile of IL-10-mediated protection during chemical colitis and which were the underlying mechanisms. Using a novel mouse model of inducible IL-10 overexpression (pMT-10), described here, we show that mice preconditioned with IL-10 for 8 days before dextran sulfate sodium (DSS) administration developed a milder colitic phenotype. In IL-10-induced colitic mice, Ly6C cells isolated from the lamina propria showed a decreased inflammatory profile. Because our mouse model leads to transcription of the IL-10 transgene in the bone marrow and elevated seric IL-10 concentration, we investigated whether IL-10 could imprint immune cells in a long-lasting way, thus conferring sustained protection to colitis. We show that this was not the case, as IL-10-afforded protection was only observed if IL-10 induction immediately preceded DSS-mediated colitis. Thus, despite the protection afforded by IL-10 in colitis, novel strategies are required, specifically to achieve long-lasting protection.
TypeArticle
URIhttp://hdl.handle.net/1822/58045
DOI10.3389/fimmu.2018.00400
ISSN1664-3224
e-ISSN1664-3224
Publisher versionhttps://www.frontiersin.org/articles/10.3389/fimmu.2018.00400/full
Peer-Reviewedyes
AccessOpen access
Appears in Collections:ICVS - Artigos em Revistas Internacionais com Referee


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