Please use this identifier to cite or link to this item:

TitleInflammatory response and long-term behavioral assessment after neonatal CO2-pneumothorax: study in a rodent model
Author(s)Miranda, Alice Augusta Macedo
Roque, Susana
Miranda, Cláudia Sofia Serre
Pêgo, José M.
Correia-Pinto, Jorge
Animals, Newborn
Carbon Dioxide
Dentate Gyrus
Disease Models, Animal
Pneumothorax, Artificial
Rats, Sprague-Dawley
Behavior, Animal
Minimally invasive surgery
CO -insufflation 2
Issue dateJul-2018
PublisherElsevier Inc
JournalJournal of Pediatric Surgery
CitationMiranda, A., Roque, S., Serre-Miranda, C., Pêgo, J. M., & Correia-Pinto, J. (2018). Inflammatory response and long-term behavioral assessment after neonatal CO2-pneumothorax: study in a rodent model. Journal of pediatric surgery, 53(7), 1318-1325
Abstract(s)Background: Carbon-dioxide (CO2)-pneumothorax during minimally invasive surgery induces well-known metabolic changes. However, little is known about its impact on the central nervous system.The aim of this work is to evaluate the acute impact of CO2-pneumothorax over central cytokine response and its long-term effect on animal behavior.Methods: This is an experimental study where neonatal Sprague-Dawley rats are submitted to CO2-pneumothorax. Peripheral and central cytokine response was evaluated 24 h after insufflation, and peripheral immune cell phenotyping was evaluated 24 h and 4 weeks post-insufflation. Progenitor cell survival was evaluated in the hippocampal dentate gyrus, and the behavioral analysis was performed in adulthood to test cognition, anxious-like, and depressive-like behavior.Results: Significantly increased 11-10 levels were observed in the cerebrospinal-fluid (CSF) of animals submitted to CO2-pneurnothorax, while no differences were found in serum. Regarding pro-inflammatory cytokines, no differences were observed in the periphery or centrally. CO2-pneumothorax event did not alter the survival of newborn cells in the hippocampal dentate gyrus, and no impact on long-term behavior was observed.Conclusions: Neonatal animals submitted to CO2-pneumothorax present acutely increased CSF IL-10 levels. The CO2 pneumothorax seems to result in no significant outcome over neurodevelopment as no functional behavioral alterations were observed in adulthood.
Publisher version
AccessEmbargoed access (3 Years)
Appears in Collections:ICVS - Artigos em Revistas Internacionais com Referee

Files in This Item:
File Description SizeFormat 
  Until 2021-08-01
1,17 MBAdobe PDFView/Open

Partilhe no FacebookPartilhe no TwitterPartilhe no DeliciousPartilhe no LinkedInPartilhe no DiggAdicionar ao Google BookmarksPartilhe no MySpacePartilhe no Orkut
Exporte no formato BibTex mendeley Exporte no formato Endnote Adicione ao seu ORCID