Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/57899

TitleInflammatory response and long-term behavioral assessment after neonatal CO2-pneumothorax: study in a rodent model
Author(s)Miranda, Alice Augusta Macedo
Roque, Susana
Miranda, Cláudia Sofia Serre
Pêgo, José M.
Correia-Pinto, Jorge
KeywordsAnimals
Animals, Newborn
Carbon Dioxide
Cytokines
Dentate Gyrus
Disease Models, Animal
Interleukin-10
Leukocytes
Male
Pneumothorax, Artificial
Rats
Rats, Sprague-Dawley
Behavior, Animal
Neonate
Minimally invasive surgery
Pneumothorax
CO2-insufflation
Inflammation
CO -insufflation 2
Issue dateJul-2018
PublisherElsevier Inc
JournalJournal of Pediatric Surgery
CitationMiranda, A., Roque, S., Serre-Miranda, C., Pêgo, J. M., & Correia-Pinto, J. (2018). Inflammatory response and long-term behavioral assessment after neonatal CO2-pneumothorax: study in a rodent model. Journal of pediatric surgery, 53(7), 1318-1325
Abstract(s)Background: Carbon-dioxide (CO2)-pneumothorax during minimally invasive surgery induces well-known metabolic changes. However, little is known about its impact on the central nervous system.The aim of this work is to evaluate the acute impact of CO2-pneumothorax over central cytokine response and its long-term effect on animal behavior.Methods: This is an experimental study where neonatal Sprague-Dawley rats are submitted to CO2-pneumothorax. Peripheral and central cytokine response was evaluated 24 h after insufflation, and peripheral immune cell phenotyping was evaluated 24 h and 4 weeks post-insufflation. Progenitor cell survival was evaluated in the hippocampal dentate gyrus, and the behavioral analysis was performed in adulthood to test cognition, anxious-like, and depressive-like behavior.Results: Significantly increased 11-10 levels were observed in the cerebrospinal-fluid (CSF) of animals submitted to CO2-pneurnothorax, while no differences were found in serum. Regarding pro-inflammatory cytokines, no differences were observed in the periphery or centrally. CO2-pneumothorax event did not alter the survival of newborn cells in the hippocampal dentate gyrus, and no impact on long-term behavior was observed.Conclusions: Neonatal animals submitted to CO2-pneumothorax present acutely increased CSF IL-10 levels. The CO2 pneumothorax seems to result in no significant outcome over neurodevelopment as no functional behavioral alterations were observed in adulthood.
TypeArticle
URIhttp://hdl.handle.net/1822/57899
DOI10.1016/j.jpedsurg.2017.08.016
ISSN0022-3468
e-ISSN1531-5037
Publisher versionhttps://www.sciencedirect.com/science/article/pii/S0022346817305079
Peer-Reviewedyes
AccessEmbargoed access (3 Years)
Appears in Collections:ICVS - Artigos em Revistas Internacionais com Referee

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