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TitleResponse surface statistical optimization of bacterial nanocellulose fermentation in static culture using a low-cost medium
Author(s)Rodrigues, Ana Cristina
Fontão, A.
Leal, Marta
Soares da Silva, F. A. G.
Coelho, Aires
Wan, Yizao
Dourado, Fernando
Gama, F. M.
KeywordsBNC production optimization
Culture medium depth
Low-cost substrates
Response surface methodology-central composite design
Surface area
Issue date25-Mar-2019
JournalNew Biotechnology
CitationRodrigues, Ana Cristina; Fontão, A.; Leal, Marta; Soares da Silva, F. A. G.; Coelho, Aires; Wan, Yizao; Dourado, Fernando; Gama, F. M., Response surface statistical optimization of bacterial nanocellulose fermentation in static culture using a low-cost medium. New Biotechnology, 49, 19-27, 2019
Abstract(s)This work aimed at the optimization of bacterial nanocellulose (BNC) production by static culture, using Komagataeibacter xylinus BPR 2001 (K. xylinus). Response surface methodology - central composite design was used to evaluate the effect of inexpensive and widely available nutrient sources, namely molasses, ethanol, corn steep liquor (CSL) and ammonium sulphate, on BNC production yield. The optimized parameters for maximum BNC production were % (m/v): molasses 5.38, CSL 1.91, ammonium sulphate 0.63, disodium phosphate 0.270, citric acid 0.115 and ethanol 1.38 % (v/v). The experimental and predicted maximum BNC production yields were 7.5±0.54g/L and 6.64±0.079g/L, respectively and the experimental and predicted maximum BNC productivity were 0.829±0.046g/L/day and 0.734±0.079g/L/day, after 9 days of static culture fermentation, at 30°C. The effect of surface area and culture medium depth on production yield and productivity were also studied. BNC dry mass production increased linearly with surface area, medium depth and fermentation time. So long as nutrients were still available in the culture media, BNC mass productivity was constant. The results show that a high BNC production yield can be obtained by static culture of K. xylinus BPR 2001 using a low-cost medium. These are promising conditions for the static industrial scale BNC production, since as compared to agitated bioreactors, higher productivities may be reached, while avoiding high capital and operating costs.
DescriptionSupplementary material related to this article can be found, in the online version, at doi:
AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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