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TitleThe MNN2 gene knockout modulates the antifungal resistance of biofilms of Candida glabrata
Author(s)Rodrigues, Célia Fortuna
Boas, Diana Patrícia Andrade Vilas
Haynes, Ken
Henriques, Mariana
KeywordsCandida glabrata
MNN2 gene
biofilm matrix
Issue date30-Oct-2018
PublisherMultidisciplinary Digital Publishing Institute (MDPI)
CitationRodrigues, Célia F.; Vilas Boas, Diana; Haynes, Ken; Henriques, Mariana, The MNN2 gene knockout modulates the antifungal resistance of biofilms of Candida glabrata. Biomolecules, 8(4), 130, 2018
Abstract(s)Candida glabrata biofilms are recognized to have high resistance to antifungals. In order to understand the effect of mannans in the resistance profile of C. glabrata mature biofilms, C. glabrata mnn2 was evaluated. Biofilm cell walls were analysed by confocal laser scanning microscopy (CLSM) and their susceptibility was assessed for fluconazole, amphotericin B, caspofungin, and micafungin. Crystal violet and Alcian Blue methods were performed to quantify the biomass and the mannans concentration in the biofilm cells and matrices, respectively. The concentration of -1,3 glucans was also measured. No visible differences were detected among cell walls of the strains, but the mutant had a high biomass reduction, after a drug stress. When compared with the reference strain, it was detected a decrease in the susceptibility of the biofilm cells and an increase of -1,3 glucans in the C. glabrata mnn2. The deletion of the MNN2 gene in C. glabrata induces biofilm matrix and cell wall variabilities that increase the resistance to the antifungal drug treatments. The rise of -1,3 glucans appears to have a role in this effect.
Publisher version
AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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