Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/5671
Registo completo
Campo DC | Valor | Idioma |
---|---|---|
dc.contributor.author | Dominguez, Ana | - |
dc.contributor.author | Santos, Isabel M. | - |
dc.contributor.author | Teixeira, J. A. | - |
dc.contributor.author | Lima, Nelson | - |
dc.date.accessioned | 2006-10-17T16:12:20Z | - |
dc.date.available | 2006-10-17T16:12:20Z | - |
dc.date.issued | 2006 | - |
dc.identifier.citation | "Revista Iberoamericana de Micología" ISSN 1130-1406. 23:5 (2006) 189-191. | eng |
dc.identifier.issn | 1130-1406 | eng |
dc.identifier.uri | https://hdl.handle.net/1822/5671 | - |
dc.description.abstract | Several microorganisms are reported to have transfructosylation activity due to fructosyltransferase and/or fructofuranosidase activities. However, the search for other fungi with higher transfructosylation activity remains a challenge. So, a presumptive and indirect colorimetric plate assay for the evaluation of transfructosylation activity in fungi was developed which involved the simultaneous determination in the same plate of glucose and fructose released from sucrose. The method entailed the (a) glucose oxidase-peroxidase coupled reaction using phenol and 4-aminoantipyrine for determination of glucose; and (b) fructose dehydrogenase oxidation in the presence of a tetrazolium salt for determination of fructose. The presence of enzymes with transfructosylation activity was identified by the formation of pink (presence of glucose) and blue (presence of fructose) halos around the fungal colony. In conclusion, the results showed that the method is suitable for screening a large number of fungi due to its simplicity, reproducibility and rapidity and also gives a relative quantitative estimation of the transfructosylation activity of different fungi species. | eng |
dc.description.sponsorship | Agência de Inovação (AdI), Portugal, project BIOFILM Fundação para a Ciência e a Tecnologia (FCT) | por |
dc.language.iso | eng | eng |
dc.publisher | Asociación Española de Micología (AEM) | eng |
dc.rights | openAccess | eng |
dc.subject | Transfructosylation | eng |
dc.subject | Fructosyltransferase | eng |
dc.subject | Fructofuranosidase | eng |
dc.subject | Fructose | eng |
dc.subject | Glucose | eng |
dc.title | New and simple plate test for screening relative transfructosylation activity of fungi | eng |
dc.type | article | por |
dc.peerreviewed | yes | eng |
sdum.number | 3 | eng |
sdum.pagination | 189-191 | eng |
sdum.publicationstatus | published | eng |
sdum.volume | 23 | eng |
oaire.citationStartPage | 189 | por |
oaire.citationEndPage | 191 | por |
oaire.citationIssue | 3 | por |
oaire.citationVolume | 23 | por |
dc.identifier.doi | 10.1016/S1130-1406(06)70042-0 | por |
dc.identifier.pmid | 17196028 | por |
sdum.journal | Revista Iberoamericana de Micología | por |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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RIM-189191[2].pdf | 82,91 kB | Adobe PDF | Ver/Abrir |