Please use this identifier to cite or link to this item:

TitleDigestion of beta-carotene loaded oleogels and transepithelial transport: in-vitro study
Author(s)Martins, Artur J.
Gonçalves, Catarina
Amado, Isabel
Pastrana, Lorenzo M.
Cunha, Rosiane
Vicente, A. A.
Cerqueira, Miguel A.
Issue date23-Oct-2018
CitationMartins, Artur J.; Gonçalves, Catarina; Amado, Isabel; Pastrana, Lorenzo M.; Cunha, Rosiane; Vicente, António A.; Cerqueira, Miguel A., Digestion of beta-carotene loaded oleogels and transepithelial transport: in-vitro study. IUFoST 2018 - World Congress of Food Science and Technology. Mumbai, India, Oct 23-27, 2018.
Abstract(s)performance as carriers of bioactives, and their behaviour under gastrointestinal conditions has been scarcely studied. This work reports the behaviour of two distinct edible oleogels, loaded with a bioactive compound under in-vitro digestion conditions and assesses the bioactive bioacessibility and bioavailability of the bioactive compound. Method(s) and Results: Two types of gelation were used for the production of oleogels (i.e. single- and multi-component gelation), using respectively beeswax and a sterol mixture of gamma-oryzanol and beta-sitosterol (60X40 ratio). For both cases, long chain triglycerides were structured with 8% (w/w) of gelator and loaded with 0.1% (w/w) of beta-carotene. The recorded average hardness of sterol-based oleogels was approximately 4-fold higher than for beeswax oleogels. In-vitro digestion of oleogels was evaluated following the INFOGEST protocol. Bioacessibility of beta-carotene was of approx. 26% for sterol-based oleogels and close to 20% for beeswax-based oleogels. Afterwards, the digested samples were placed in contact with a monolayer of differentiated Caco-2 and HT-29-MTX cells, to determine the transepithelial transport of beta-carotene (bioavailability). Sterol gels favor the bioavailability of beta-carotene with an increase of 15% of bioactive transport over beeswax-based oleogels. No cytotoxicity of oleogels was detected. Conclusions: This study shows that the INFOGEST standard protocol is suitable for the in-vitro digestion evaluation of oleogels and that the bioacessibility of beta-carotene in these systems lays between 20 and 26 %. Oleogels are able to carry beta-carotene through the digestive process being enhanced by sterol gels.
Publisher version
AccessOpen access
Appears in Collections:CEB - Painéis em Conferências / Posters in Conferences

Files in This Item:
File Description SizeFormat 
document_49067_1.pdf2,78 MBAdobe PDFView/Open

Partilhe no FacebookPartilhe no TwitterPartilhe no DeliciousPartilhe no LinkedInPartilhe no DiggAdicionar ao Google BookmarksPartilhe no MySpacePartilhe no Orkut
Exporte no formato BibTex mendeley Exporte no formato Endnote Adicione ao seu ORCID