Utilize este identificador para referenciar este registo: http://hdl.handle.net/1822/56676

TítuloBiochemical gradients to generate 3D heterotypic-like tissues with isotropic and anisotropic architectures
Autor(es)Canadas, Raphael Faustino
Ren, Tachen
Marques, A. P.
Oliveira, J. M.
Reis, R. L.
Demirci, Utkan
RevistaAdvanced Functional Materials
CitaçãoCanadas R. F., Ren T., Marques A. P., Oliveira J. M., Reis R. L., Demirci U. Biochemical Gradients to Generate 3D Heterotypic-Like Tissues with Isotropic and Anisotropic Architectures, Advanced Functional Materials, Issue 1804148, doi: 10.1002/adfm.201804148, 2018
Resumo(s)Anisotropic 3D tissue interfaces with functional gradients found in nature are replicated in vitro for drug development and tissue engineering. Even though different fabrication techniques, based on material science engineering and microfluidics, are used to generate such microenvironments, mimicking the native tissue gradient is still a challenge. Here, the fabrication of 3D structures are described with linear/random porosity and gradient distribution of hydroxyapatite microparticles which are combined with a gradient of growth factors generated by a dual chamber for the development of heterotypicâ like tissues. The hydroxyapatite gradient is formed by applying a thermal ramp from the first to the second gel layer, and the porous architecture is controlled through ice templating. A 3D osteochondral (OC) tissue model is developed by codifferentiating fat pad adiposeâ derived stem cells. Osteogenic and chondrogenic markers expression is spatially controlled, as it occurs in the native osteochondral unit. Additionally, a prevasculature is spatially induced by the perfusion of proangiogenic medium in the boneâ like region, as observed in the native subchondral bone. Thus, in this study, precise spatial control is developed over cell/tissue phenotype and formation of prevasculature which opens up possibilities for the study of complex tissues interfaces, with broader applications in drug testing and regenerative medicine.
Versão da editorahttps://onlinelibrary.wiley.com/doi/abs/10.1002/adfm.201804148
Arbitragem científicayes
AcessoembargoedAccess (2 Years)
Aparece nas coleções:3B’s - Artigos em revistas/Papers in scientific journals

Ficheiros deste registo:
Ficheiro Descrição TamanhoFormato 
19653-Canadas_et_al-2018-Advanced_Functional_Materials.pdf12,09 MBAdobe PDFVer/Abrir  Solicitar cópia ao autor!

Partilhe no FacebookPartilhe no TwitterPartilhe no DeliciousPartilhe no LinkedInPartilhe no DiggAdicionar ao Google BookmarksPartilhe no MySpacePartilhe no Orkut
Exporte no formato BibTex mendeley Exporte no formato Endnote Adicione ao seu Currículo DeGóis