Utilize este identificador para referenciar este registo: http://hdl.handle.net/1822/56620

TítuloDownscale fermentation for xylooligosaccharides production by recombinant Bacillus subtilis 3610
Autor(es)Amorim, Cláudia
Silvério, Sara C.
Gonçalves, Raquel F. S.
Pinheiro, Ana Cristina
Silva, Soraia
Coelho, Elisabete
Coimbra, Manuel A.
Prather, Kristala L. J.
Rodrigues, L. R.
Palavras-chaveXylooligosaccharides
Bacillus subtilis
prebiotic
xylan
xylanase
DataFev-2019
EditoraElsevier
RevistaCarbohydrate Polymers
CitaçãoAmorim, Cláudia; Silvério, Sara C.; Gonçalves, Raquel F. S.; Pinheiro, Ana Cristina; Silva, Soraia; Coelho, Elisabete; Coimbra, Manuel A.; Prather, Kristala L. J.; Rodrigues, Lígia R., Downscale fermentation for xylooligosaccharides production by recombinant Bacillus subtilis 3610. Carbohydrate Polymers, 205, 176-183, 2019
Resumo(s)The global demand of prebiotics such as xylooligosaccharides (XOS) has been growing over the years, motivating the search for different production processes with increased efficiency. In this study, a cloned Bacillus subtilis 3610, containing the xylanase gene xyn2 of Trichoderma reesei coupled with an endogenous secretion tag, was selected for XOS production through direct fermentation of beechwood xylan. A mixture of XOS with a degree of polymerization ranging from 4-6 was obtained, presenting high stability after a static in vitro digestion (98.5±0.2%). The maximum production yield expressed as total XOS per amount of xylan (306±4mg/g) was achieved after 8h of fermentation operating under one-time impulse fed-batch. The optimal conditions found were pH 6.0 and 42.5°C, using 2.5g/L of initial concentration of xylan increased up to 5.0g/L at 3h. Xylopentaose was the major oligosaccharide produced, representing 47% of the total production yield.
Tipoarticle
DescriçãoSupplementary material related to this article can be found, in the online version, at doi:https://doi.org/10.1016/j.carbpol.2018.09.088.
URIhttp://hdl.handle.net/1822/56620
DOI10.1016/j.carbpol.2018.09.088
ISSN0144-8617
e-ISSN0144-8617
Versão da editorahttp://www.journals.elsevier.com/carbohydrate-polymers/
Arbitragem científicayes
AcessoopenAccess
Aparece nas coleções:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

Ficheiros deste registo:
Ficheiro Descrição TamanhoFormato 
document_48969_1.pdf457,08 kBAdobe PDFVer/Abrir

Partilhe no FacebookPartilhe no TwitterPartilhe no DeliciousPartilhe no LinkedInPartilhe no DiggAdicionar ao Google BookmarksPartilhe no MySpacePartilhe no Orkut
Exporte no formato BibTex mendeley Exporte no formato Endnote Adicione ao seu Currículo DeGóis