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TitleIN-SITU, real-time bioreactor monitoring by fiber optics sensors
Author(s)Silva, Rui
Silva, João S.
Vicente, A. A.
Teixeira, J. A.
Martins, R.
KeywordsUV-VIS-SWNIR spectroscopy
Real-time monitoring
Multivariate calibration
Multivariate calibrarion
Issue date2009
PublisherInstitute for Systems and Technologies of Information, Control and Communication (INSTICC)
CitationSilva, Rui; Silva, João S.; Vicente, António A.; Teixeira, José A.; Martins, R., IN-SITU, real-time bioreactor monitoring by fiber optics sensors. BIOSIGNALS 2009 - Proceedings of the 2nd International Conference on Bio-Inspired Systems and Signal Processing. Porto, Portugal, Jan 14-17, 327-336, 2009. ISBN: 978-989-8111-65-4
Abstract(s)One of the most studied bioprocesses is fermentation by yeasts. Although this is true, there is still the lack of real-time instrumentation that is capable of providing detailed information on metabolic state of fermentations. In this research we explore the possibility of using UV-VIS-SWNIR spectroscopy as a high-output, non-destructive and multivariate methodology of monitoring beer fermentation. We herein report the implementation of the a fibber optics sensor and the capacity for detecting key parameters by partial least squares regression for biomass, extract, pH and total sugars. Results show that UV-VIS-SWNIR is a robust technique for monitoring beer fermentations, being able to provide detailed information spectroscopic fingerprinting of the process. Calibrations were possible to obtain for all the studied parameters with R2 of 0.85 to 0.94 in the UV-VIS region and 0.95 to 0.97 in the VIS-SWNIR region. This preliminary study allowed to conclude that further improvements in experimental methodology and signal processing may turn this technique into a valuable instrument for detailed metabolic studies in biotechnology.
TypeConference paper
AccessOpen access
Appears in Collections:CEB - Artigos em Livros de Atas / Papers in Proceedings

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