Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/56382

TitleUnveiling the fate of adhering bacteria to antimicrobial surfaces: expression of resistance-associated genes and macrophage-mediated phagocytosis
Author(s)Alves, Diana
Magalhães, Andreia P.
Neubauer, Damian
Bauer, Marta
Kamysz, Wojciech
Pereira, Maria Olívia
Keywordsmicrobial resistance
antimicrobial coatings
macrophages
biomaterial-associated infections
Issue date15-Sep-2018
PublisherElsevier
JournalActa Biomaterialia
CitationAlves, Diana; Magalhães, Andreia P.; Neubauer, Damian; Bauer, Marta; Kamysz, Wojciech; Pereira, Maria Olívia, Unveiling the fate of adhering bacteria to antimicrobial surfaces: expression of resistance-associated genes and macrophage-mediated phagocytosis. Acta Biomaterialia, 78, 189-197, 2018
Abstract(s)Since most antibacterial coatings reported to fight biomaterial-associated infections (BAI) fail in completely preventing bacterial colonization, it is crucial to know the impact of that small fraction of adhered bacteria in BAI recrudescence. This study aims to understand the fate of Staphylococcus aureus able to adhere to an antimicrobial coating previously developed, in terms of potential development of bacterial resistance and their macrophage-mediated phagocytosis. Antimicrobial coating comprised the co-immobilization of Palm peptide and DNase I onto polydimethylsiloxane. Expression of genes associated to resistance and virulence mechanisms showed that cells in contact with antimicrobial surfaces for a long period of 30 days, exhibit genes equally or less expressed, as compared to cells recovered from control surfaces. Recovered cells also exhibit the same susceptibility patterns, which strengthens the evidence of no resistance development. Remarkably, cells adhered to modified surfaces shows a reduced metabolic activity upon vancomycin treatment unlike the cells found on control surfaces, which can be identified as a clinical opportunity for prophylactically administration after implant surgery. Furthermore, results highlight that functionalization of PDMS with Palm and DNase I should not compromise the action of host immune cells. The overall results reinforce the potential of this antimicrobial strategy to fight BAI.
TypeArticle
URIhttp://hdl.handle.net/1822/56382
DOI10.1016/j.actbio.2018.07.052
ISSN1742-7061
Publisher versionhttp://www.journals.elsevier.com/acta-biomaterialia/
Peer-Reviewedyes
AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

Files in This Item:
File Description SizeFormat 
document_47882_1.pdf1,23 MBAdobe PDFView/Open

Partilhe no FacebookPartilhe no TwitterPartilhe no DeliciousPartilhe no LinkedInPartilhe no DiggAdicionar ao Google BookmarksPartilhe no MySpacePartilhe no Orkut
Exporte no formato BibTex mendeley Exporte no formato Endnote Adicione ao seu ORCID