Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/56350

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dc.contributor.authorBotelho, C. M.por
dc.contributor.authorDinis, Hugo Daniel Costapor
dc.contributor.authorToubarro, Duartepor
dc.contributor.authorSimões, Nelsonpor
dc.contributor.authorTeixeira, J. A.por
dc.date.accessioned2018-10-18T11:20:53Z-
dc.date.available2018-10-18T11:20:53Z-
dc.date.issued2018-09-09-
dc.identifier.citationBotelho, Cláudia M.; Dinis, H.; Toubarro, Duarte; Simões, Nelson; Teixeira, José A., Keratin bioactive peptides for the treatment of skin disorders. ESBES 2018 - 12th European Symposium on Biochemical Engineering Sciences. Lisbon, Portugal, Sep 9-12, 2018.por
dc.identifier.urihttp://hdl.handle.net/1822/56350-
dc.description.abstract[Excerpt] Several skin disorders, like chronic wounds and psoriasis involve several steps for skin regeneration. On wound repair keratinocyte migration for reepithelialization is required. Psoriatic lesions result from the interaction between hyperproliferative keratinocytes, inflammatory cells and immune cells. Chicken feather (CF) are composed by keratin and its disposal is a critical problem. It is possible to hydrolyze (CF) to obtain high value keratin peptides minimizing the impact on the environment and giving value to otherwise waste material. [...]-
dc.description.sponsorshipSeveral skin disorders, like chronic wounds and psoriasis involve several steps for skin regeneration. On wound repair keratinocyte migration for reepithelialization is required. Psoriatic lesions result from the interaction between hyperproliferative keratinocytes, inflammatory cells and immune cells. Chicken feather (CF) are composed by keratin and its disposal is a critical problem. It is possible to hydrolyze (CF) to obtain high value keratin peptides minimizing the impact on the environment and giving value to otherwise waste material. Keratin peptides (KP) were obtained by submer ged fermentation using a Bacillus cereus strain (S188D) growing on keratin. Briefly , 1 g of CF was incubated with 100 mL of growing media at 30ºC and 200 rpm for 48h. The KP were subjected to dif ferent purification procedures: i) isolated using a C18 column (stage1); ii) eluted using a cut- off of 5kDA and fractioned with dif ferent percentages of acetonitrile 20% and 40% (stage2); iii) each fraction was eluted using a Superdex peptide column to obtain dif ferent chromatographic picks, P1, P2, P3, P4, and P5 (stage3). The keratinocytes cells were grown in DMEM supplement with 10% of FBS at 37ºC and 5% of CO 2 . Cells were grown till confluence and a scratch was performed; 45 mg/mL of the KP , from the 3 stages of purification were placed in contact with the cells for 24h. When the stage1 KP were in contact with the cells there was a decrease on cell migration in comparison to the control. The second stage peptides (20% and 40%) did not induce any significant changes on the cell migration, but the presence of the peptides significantly decrease cell proliferation in 43% and 54%, respectively . An opposite result was observed for the third stage peptides, S188D 20% P1, P2, P3 and S188D 40% P3, seems to increase cell migration, while S188D P4 and P5 significantly increased cell migration in 20% and 19%, respectively . Only S188D 40% P2 significantly increased cell proliferation. Depending on the fraction of KP it is possible to obtain opposite ef fects regarding cell migration. It is possible to modulate the cellular response of the keratinocytes, by using simple fractioning. The KP used on this study were sequenced and the mechanisms that behind these results are being evaluatedpor
dc.language.isoengpor
dc.rightsopenAccesspor
dc.titleKeratin bioactive peptides for the treatment of skin disorderspor
dc.typeconferenceAbstractpor
dc.peerreviewedyespor
dc.relation.publisherversionhttps://esbes2018.org/por
dc.commentsCEB48944por
oaire.citationConferenceDate9 Set. - 12 Set. 2018por
sdum.event.title12th Symposium of the European Society of Biochemical Engineering Sciencespor
sdum.event.typeotherpor
oaire.citationConferencePlaceLisbon, Portugalpor
dc.date.updated2018-10-18T10:06:18Z-
dc.subject.fosCiências Médicas::Biotecnologia Médicapor
dc.description.publicationversioninfo:eu-repo/semantics/publishedVersionpor
sdum.conferencePublication12th Symposium of the European Society of Biochemical Engineering Sciencespor
Appears in Collections:CEB - Resumos em Livros de Atas / Abstracts in Proceedings

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