Utilize este identificador para referenciar este registo: http://hdl.handle.net/1822/56340

TítuloBioreactors and microfluidics for osteochondral interface maturation
Autor(es)Canadas, Raphael Faustino
Marques, A. P.
Reis, R. L.
Oliveira, J. M.
Palavras-chaveBioreactors
Dynamic systems
Microfluidics
Osteochondral tissue engineering
Data2018
EditoraSpringer
RevistaAdvances in Experimental Medicine and Biology
CitaçãoCanadas R. F., Marques A. P., Reis R. L., Oliveira J. M. Bioreactors and Microfluidics for Osteochondral Interface Maturation, Osteochondral Tissue Engineering, pp. 395-420, doi:10.1007/978-3-319-76735-2_18, 2018
Resumo(s)The cell culture techniques are in the base of any biology-based science. The standard techniques are commonly static platforms as Petri dishes, tissue culture well plates, T-flasks, or well plates designed for spheroids formation. These systems faced a paradigm change from 2D to 3D over the current decade driven by the tissue engineering (TE) field. However, 3D static culture approaches usually suffer from several issues as poor homogenization of the formed tissues and development of a necrotic center which limits the size of in vitro tissues to hundreds of micrometers. Furthermore, for complex tissues as osteochondral (OC), more than recovering a 3D environment, an interface needs to be replicated. Although 3D cell culture is already the reality adopted by a newborn market, a technological revolution on cell culture devices needs a further step from static to dynamic already considering 3D interfaces with dramatic importance for broad fields such as biomedical, TE, and drug development. In this book chapter, we revised the existing approaches for dynamic 3D cell culture, focusing on bioreactors and microfluidic systems, and the future directions and challenges to be faced were discussed. Basic principles, advantages, and challenges of each technology were described. The reported systems for OC 3D TE were focused herein.
TipobookPart
URIhttp://hdl.handle.net/1822/56340
ISBN978-3-319-76735-2
DOI10.1007/978-3-319-76735-2_18
ISSN0065-2598
Versão da editorahttps://link.springer.com/chapter/10.1007/978-3-319-76735-2_18#citeas
Arbitragem científicayes
AcessoembargoedAccess (2 Years)
Aparece nas coleções:3B’s - Capítulos de Livros/Book Chapters

Ficheiros deste registo:
Ficheiro Descrição TamanhoFormato 
19618-Chapter 26_Bioreactors and microfluidics.pdf1,06 MBAdobe PDFVer/Abrir  Solicitar cópia ao autor!

Partilhe no FacebookPartilhe no TwitterPartilhe no DeliciousPartilhe no LinkedInPartilhe no DiggAdicionar ao Google BookmarksPartilhe no MySpacePartilhe no Orkut
Exporte no formato BibTex mendeley Exporte no formato Endnote Adicione ao seu Currículo DeGóis