Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/56340

TitleBioreactors and microfluidics for osteochondral interface maturation
Author(s)Canadas, Raphael Faustino
Marques, A. P.
Reis, R. L.
Oliveira, J. M.
KeywordsBioreactors
Dynamic systems
Microfluidics
Osteochondral tissue engineering
Issue date2018
PublisherSpringer
JournalAdvances in Experimental Medicine and Biology
CitationCanadas R. F., Marques A. P., Reis R. L., Oliveira J. M. Bioreactors and Microfluidics for Osteochondral Interface Maturation, Osteochondral Tissue Engineering, pp. 395-420, doi:10.1007/978-3-319-76735-2_18, 2018
Abstract(s)The cell culture techniques are in the base of any biology-based science. The standard techniques are commonly static platforms as Petri dishes, tissue culture well plates, T-flasks, or well plates designed for spheroids formation. These systems faced a paradigm change from 2D to 3D over the current decade driven by the tissue engineering (TE) field. However, 3D static culture approaches usually suffer from several issues as poor homogenization of the formed tissues and development of a necrotic center which limits the size of in vitro tissues to hundreds of micrometers. Furthermore, for complex tissues as osteochondral (OC), more than recovering a 3D environment, an interface needs to be replicated. Although 3D cell culture is already the reality adopted by a newborn market, a technological revolution on cell culture devices needs a further step from static to dynamic already considering 3D interfaces with dramatic importance for broad fields such as biomedical, TE, and drug development. In this book chapter, we revised the existing approaches for dynamic 3D cell culture, focusing on bioreactors and microfluidic systems, and the future directions and challenges to be faced were discussed. Basic principles, advantages, and challenges of each technology were described. The reported systems for OC 3D TE were focused herein.
TypeBook part
URIhttp://hdl.handle.net/1822/56340
ISBN978-3-319-76735-2
DOI10.1007/978-3-319-76735-2_18
ISSN0065-2598
Publisher versionhttps://link.springer.com/chapter/10.1007/978-3-319-76735-2_18#citeas
AccessEmbargoed access (2 Years)
Appears in Collections:3B’s - Capítulos de Livros/Book Chapters

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