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|Title:||Gellan Gum-based luminal fillers for peripheral nerve regeneration: an in vivo study in the rat sciatic nerve repair model|
|Author(s):||Carvalho, C. R.|
Cengiz, I. F.
Reis, R. L.
Oliveira, J. M.
Peripheral Nerve Regeneration
|Publisher:||Royal Society of Chemistry|
|Citation:||Carvalho C. R., Wrobel S., Meyer C., Branderberger C., Cengiz I. F., López-Cebral R., Silva-Correia J., Ronchi J., Reis R. L., Grothe C., Oliveira J. M., Haastert-Talini K. Gellan Gum-based luminal fillers for peripheral nerve regeneration: an in vivo study in the rat sciatic nerve repair model, BIOMATERIALS SCIENCE, Vol. 6, Issue 6, pp. 1059-1075, doi:10.1039/c7bm01101f, 2018|
|Abstract(s):||Peripheral nerve injuries (PNI) resulting in a gap to be bridged between the transected nerve ends are commonly reconstructed with autologous nerve tissue, but there is a need for valuable alternatives. This experimental work considers the innovative use of the biomaterial Gellan Gum (GG) as a luminal ﬁller for nerve guidance channels made from chitosan with a 5% degree of acetylation. The engineered constructs should remodel the structural support given to regenerating axons by the so-called bands of Büngner. Four diﬀerent GG formulations were produced by combining varying amounts of High-Acyl GG (HA-GG) and Methacrylated GG (MA-GG). The eﬀective porosity of the freeze-dried networks was analysed by SEM and micro-CT 3D reconstructions, while the degradation and swelling abilities were characterized in vitro for up to 30 days. The metabolic activity and viability of immortalized Schwann cells seeded onto the freeze-dried networks were also evaluated. Finally, the developed hydrogel formulations were freezedried within the chitosan nerve guides and implanted in a 10 mm rat sciatic nerve defect. Functional and histomorphological analyses after 3, 6, and 12 weeks in vivo revealed that although it did not result in improved nerve regeneration, the NGC25:75 formulations could provide a basis for further development of GG scaﬀolds as luminal ﬁllers for hollow nerve guidance channels.|
|Appears in Collections:||3B’s - Artigos em revistas/Papers in scientific journals|
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