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|Title:||Isolation and chemical characterization of chondroitin sulfate from cartilage by-products of Blackmouth Catshark (Galeus melastomus)|
|Author(s):||José, V. A.|
Reis, R. L.
Antelo, Luis, T.
Pérez-Martín, R. I.
|Keywords:||chondroitin sulfate production|
cartilage Galeus melastomus by-products
molecular weight glycosaminoglycans determination
bycatch waste management
|Citation:||José V. A., Fraguas J., Novoa-Carballal R., Reis R. L., Antelo Luis T., Pérez-Martín R. I., Valcarcel J. Isolation and Chemical Characterization of Chondroitin Sulfate from Cartilage By-Products of Blackmouth Catshark (Galeus melastomus), Marine Drugs, Vol. 16, Issue 10, pp. 344, doi: 10.3390/md16100344, 2018.|
|Abstract(s):||Chondroitin sulfate (CS) is a glycosaminoglycan actively researched for pharmaceutical, nutraceutical and tissue engineering applications. CS extracted from marine animals displays different features from common terrestrial sources, resulting in distinct properties, such as anti-viral and anti-metastatic. Therefore, exploration of undescribed marine species holds potential to expand thepossibilitiesofcurrently-knownCS.Accordingly,wehavestudiedfortheﬁrsttimetheproduction and characterization of CS from blackmouth catshark (Galeusmelastomus), a shark species commonly discarded as by-catch. The process of CS puriﬁcation consists of cartilage hydrolysis with alcalase, followed by two different chemical treatments and ending with membrane puriﬁcation. All steps were optimized by response surface methodology. According to this, the best conditions for cartilage proteolysis were established at 52.9 ◦C and pH = 7.31. Subsequent puriﬁcation by either alkaline treatment or hydroalcoholic alkaline precipitation yielded CS with purities of 81.2%, 82.3% and 97.4% respectively, after 30-kDa membrane separation. The molecular weight of CS obtained ranges 53–66 kDa, depending on the conditions. Sulfation proﬁles were similar for all materials, with dominant CS-C (GlcA-GalNAc6S) units (55%), followed by 23–24% of CS-A (GlcA-GalNAc4S), a substantial amount (15–16%) of CS-D (GlcA2S-GalNAc6S) and less than 7% of other disulfated and unsulfated disaccharides.|
|Appears in Collections:||3B’s - Artigos em revistas/Papers in scientific journals|
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