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|Title:||Fish sarcoplasmic proteins as a high value marine material for wound dressing applications|
|Author(s):||Vieira, S. F.|
Franco, A. R.
Fernandes, E. M.
Pires, R. A.
Reis, R. L.
Neves, N. M.
|Journal:||Colloids and Surfaces B: Biointerfaces|
|Citation:||Vieira S. F., Franco A. R., Fernandes E. M., Amorim S., Ferreira H., Pires R. A., Reis R. L., Martins A., Neves N. M. Fish sarcoplasmic proteins as a high value marine material for wound dressing applications, Colloids and Surfaces B: Biointerfaces, Vol. 167, pp. 310-317, doi:10.1016/j.colsurfb.2018.04.002, 2018|
|Abstract(s):||Fish sarcoplasmic proteins (FSP) constitute around 25–30% of the total fish muscle protein. As the FSP are water soluble, FSP were isolated from fresh cod (Gadus morhua) by centrifugation. By SDS-PAGE, it was possible to determine the composition of FSP extracts (FSP-E). The FSP-E undergo denaturation at 44.12 ± 2.34° C, as characterized by differential scanning calorimetry thermograms (DSC). The secondary structure of FSP-E is mainly composed by α-helix structure, as determined by circular dichroism. The cytocompatibility of FSP-E, at concentrations ranging from 5 to 20 mg/mL, was investigated. Concentrations lower than 10 mg/mL have no cytotoxicity cultures of fibroblasts over 72 h. Further on, FSP membranes (FSP-M) were produced by spin coating to evaluate its properties. FSP-M shown having uniform surface as analyzed by Scanning Electron Microscopy (SEM). The relative amount of α-helix structures is higher when compared with the FSP-E. The FSP-M have higher temperature stability than the FSP-E, since they presented a denaturation temperature of 58.88 ± 3.36° C, according to the DSC analysis. FSP-M shown distinctive mechanical properties, with a stiffness of 16.57 ± 3.95 MPa and a yield strength of 23.85 ± 5.97 MPa. Human lung fibroblasts cell lines (MRC-5) were cultured in direct contact with FSP-M, demonstrating its cytocompatibility for 48 h. Based on these results, FSP can be considered a potential biomaterial recovered from nature, for wound dressing applications.|
|Access:||embargoedAccess (1 Year)|
|Appears in Collections:||3B’s - Artigos em revistas/Papers in scientific journals|
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