Utilize este identificador para referenciar este registo: http://hdl.handle.net/1822/56285

TítuloKefiran biopolymer: Evaluation of its physicochemical and biological properties
Autor(es)Radhouani, H.
Gonçalves, C.
Maia, F. R.
Oliveira, J. M.
Reis, R. L.
Palavras-chavecytotoxicity
Drug Delivery System
enzymatic resistance
Exopolysaccharide Kefiran
physicochemical characterization
Portuguese kefir grains
DataAgo-2018
EditoraSAGE
RevistaJournal of Bioactive and Compatible Polymers
CitaçãoRadhouani H., Gonçalves C., Maia F. R., Oliveira J. M., Reis R. L. Kefiran biopolymer: Evaluation of its physicochemical and biological properties, Journal Of Bioactive And Compatible Polymers, Vol. 33, Issue 5, pp. 461-478, doi:10.1177/0883911518793914, 2018
Resumo(s)Kefiran, an exopolysaccharide produced by lactic acid bacteria, has received a great interest due to a variety of health claims. In this study, we aim to investigate the physicochemical and biological properties of Kefiran polysaccharide extracted from Portuguese kefir grains. The kefir growth rate was about 56% (w/w) at room temperature and the kefir pH after 24 h was about 4.6. The obtained yield of Kefiran polysaccharide extracted from the kefir grains was about 4.26% (w/w). The Kefiran structural features were showed in the 1H nuclear magnetic resonance spectrum. The bands observed in the infrared spectrum confirmed that the Kefiran had a β-configuration; and the X-ray photoelectron spectroscopy analysis confirmed the structure and composition of Kefiran and revealed a C/O atomic ratio of 1.46. Moreover, Kefiran showed an average molecular weight (Mw) of 534 kDa and a number-average molecular weight (Mn) of 357 kDa. Regarding the rheological data obtained, Kefiran showed an interesting adhesive performance accompanied by a pseudoplastic behavior, and the extrusion force of Kefiran was 1 N. Furthermore, Kefiran exhibited a higher resistance to hyaluronidase degradation than hyaluronic acid. Finally, Kefiran showed a lack of cytotoxic response through its ability to support metabolic activity and proliferation of L929 cells, and had no effect on these cells’ morphology. Our research suggested that Kefiran polymer has attractive and interesting properties for a wide range of biomedical applications, such as tissue engineering and regenerative medicine.
Tipopreprint
URIhttp://hdl.handle.net/1822/56285
DOI10.1177/0883911518793914
ISSN0883-9115
Versão da editorahttp://journals.sagepub.com/doi/abs/10.1177/0883911518793914?journalCode=jbca
Arbitragem científicayes
AcessoopenAccess
Aparece nas coleções:3B’s - Artigos em revistas/Papers in scientific journals

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