Utilize este identificador para referenciar este registo: http://hdl.handle.net/1822/56281

TítuloNatural deep eutectic systems as alternative nontoxic cryoprotective agents
Autor(es)Castro, V.
Craveiro, R.
Silva, J. M.
Reis, R. L.
Paiva, A.
Duarte, A. R. C.
Palavras-chaveCryoprotectants
Freeze-thawing
Natural deep eutectic solvents
Sugars
DataJun-2018
EditoraElsevier
RevistaCryobiology
CitaçãoCastro V., Craveiro R., Silva J. M., Reis R. L., Paiva A., Duarte A. R. C. Natural deep eutectic systems as alternative nontoxic cryoprotective agents, Cryobiology, Vol. 83, pp. 15-26, doi:10.1016/j.cryobiol.2018.06.010, 2018
Resumo(s)Natural deep eutectic systems (NADES) are mostly composed of natural primary metabolites such as sugars, sugar alcohols, organic acids, amino acids and amines. These simple molecules have been identified in animals living in environments with extreme temperature amplitudes, being responsible for their survival at negative temperatures during winter. Herein, we report for the first time the use of NADES based on trehalose (Treh) and glycerol (Gly) in cryopreservation, as cryoprotective agents (CPA). The evaluation of the thermal behaviour of these eutectic systems, showed that NADES have a strong effect on the water crystallization/freezing and melting process, being able to reduce the number of ice crystals and hence ice crystal damage in cells, which is a crucial parameter for their survival, upon freezing. Using this NADES as CPA, it is possible to achieve similar or even better cellular performance when compared with the gold standard for cryopreservation dimethyl sulfoxide (DMSO). In this sense, this work relates the physical properties of the NADES with their biological performance in cryopreservation. Our comprehensive strategy results in the demonstration of NADES as a promising nontoxic green alternative to the conventional CPA's used in cryopreservation methods.
Tipoarticle
URIhttp://hdl.handle.net/1822/56281
DOI10.1016/j.cryobiol.2018.06.010
ISSN10902392
Versão da editorahttps://linkinghub.elsevier.com/retrieve/pii/S0011224018301329
Arbitragem científicayes
AcessorestrictedAccess
Aparece nas coleções:3B’s - Artigos em revistas/Papers in scientific journals

Ficheiros deste registo:
Ficheiro Descrição TamanhoFormato 
19606-Castro, V.I.B., Cryobiology (2018), doi.org10.1016j.cryobiol.2018.06.010.pdf3,2 MBAdobe PDFVer/Abrir  Solicitar cópia ao autor!

Partilhe no FacebookPartilhe no TwitterPartilhe no DeliciousPartilhe no LinkedInPartilhe no DiggAdicionar ao Google BookmarksPartilhe no MySpacePartilhe no Orkut
Exporte no formato BibTex mendeley Exporte no formato Endnote Adicione ao seu Currículo DeGóis