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TitleTriggering the activation of Activin A type II receptor in human adipose stem cells towards tenogenic commitment using mechanomagnetic stimulation
Author(s)Gonçalves, A. I.
Rotherham, M.
Markides, H.
Rodrigues, M. T.
Reis, R. L.
Gomes, M. E.
Haj, A. J.
KeywordsActivin A receptor
Human adipose derived stem cells
Magnetic nanoparticles
Tendon Tissue Engineering
TGF-β/Smad2/3 signaling pathway
Activin A receptor
TGF-beta/Smad2/3 signaling pathway
Issue dateJun-2018
JournalNanomedicine: Nanotechnology, Biology and Medicine
CitationGonçalves A. I., Rotherham M., Markides H., Rodrigues M. T., Reis R. L., Gomes M. E., Haj A. E. Triggering the activation of Activin A type II receptor in human adipose stem cells towards tenogenic commitment using mechanomagnetic stimulation, Nanomedicine: Nanotechnology, Biology and Medicine, Vol. 14, Issue 4, pp. 1149-1159, doi:10.1016/j.nano.2018.02.008, 2018
Abstract(s)Stem cell therapies hold potential to stimulate tendon regeneration and homeostasis, which is maintained in response to the native mechanical environment. Activins are members of the mechano-responsive TGF-β superfamily that participates in the regulation of several downstream biological processes. Mechanosensitive membrane receptors such as activin can be activated in different types of stem cells via magnetic nanoparticles (MNPs) through remote magnetic actuation resulting in cell differentiation. In this work, we target the Activin receptor type IIA (ActRIIA) in human adipose stem cells (hASCs), using anti-ActRIIA functionalized MNPs, externally activated through a oscillating magnetic bioreactor. Upon activation, the phosphorylation of Smad2/3 is induced allowing translocation of the complex to the nucleus, regulating tenogenic transcriptional responses. Our study demonstrates the potential remote activation of MNPs tagged hASCs to trigger the Activin receptor leading to tenogenic differentiation. These results may provide insights toward tendon regeneration therapies.
Publisher version
AccessOpen access
Appears in Collections:3B’s - Artigos em revistas/Papers in scientific journals

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