Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/56279

TitleAn integral and sustainable valorisation strategy of squid pen by-products
Author(s)José, V. A.
Ramos, P.
Valcarcel, J.
Antelo, Luis, T.
Novoa-Carballal, R.
Reis, R. L.
Pérez Martín, R. I.
KeywordsChitosan
squid pens
Chitin
Loligo opalescens pen
Marine-squid peptones
Integral valorisation
Sustainability
Issue dateJul-2018
PublisherElsevier
JournalJournal Of Cleaner Production
CitationJosé V. A., Ramos P., Valcarcel J., Antelo Luis T., Novoa-Carballal R., Reis R. L., Pérez Martín R. I. An integral and sustainable valorisation strategy of squid pen by-products, Journal Of Cleaner Production, Vol. 201, pp. 207-218, doi:10.1016/j.jclepro.2018.07.316, 2018
Abstract(s)The aim of this study was to present a sustainable valorisation approach of Loligo opalescens squid pen by-products by optimal production of chitin and chitosan biopolymers and recycling of the generated effluents. The integral recovery of squid pens started with the optimization of the deproteinization process to isolate chitin, including less hazardous enzymatic alternatives to conventional chemical treatments. Response surface methodology analysis yielded optimal conditions of 53.2 °C/pH 8.84 for Alcalase, 54.7 °C/pH 9.16 for Esperase and 0.2 M NaOH/27.5 °C for alkaline hydrolysis. The generated effluents were a good source of organic nitrogen (squid peptones) for the culture of a lactic acid bacterium (Pediococcus acidilactici), with biomass and metabolite productions comparable to commercial Man, Rogosa, Sharpe medium. Secondly, optimal process time and alkali concentration for the chemical deacetylation of chitin were established at 16 h/64% NaOH, obtaining high purity chitosan (deacetylation degrees >92%, molecular weights -Mn- ranging from 139 to 300 kDa). Optimized chitin and chitosan processes led to lower energetic costs. Alkaline effluents from chitosan production were applied as a reagent in the selective precipitation of chondroitin sulphate from enzymatic hydrolysates of shark cartilage by-products. Finally, the goodness of the proposed valorisation strategy was evaluated by developing a Material Flow Analysis.
TypeArticle
URIhttp://hdl.handle.net/1822/56279
DOI10.1016/j.jclepro.2018.07.316
ISSN0959-6526
Publisher versionhttps://doi.org/10.1016/j.jclepro.2018.07.316
Peer-Reviewedyes
AccessRestricted access (UMinho)
Appears in Collections:3B’s - Artigos em revistas/Papers in scientific journals

Files in This Item:
File Description SizeFormat 
19623-Squid_Pen_CS.pdf
  Restricted access
2 MBAdobe PDFView/Open

Partilhe no FacebookPartilhe no TwitterPartilhe no DeliciousPartilhe no LinkedInPartilhe no DiggAdicionar ao Google BookmarksPartilhe no MySpacePartilhe no Orkut
Exporte no formato BibTex mendeley Exporte no formato Endnote Adicione ao seu ORCID