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dc.contributor.authorMaia, F. R.por
dc.contributor.authorDavid, M.por
dc.contributor.authorNaot, D.por
dc.contributor.authorda silva, L.por
dc.contributor.authorBastos, A. R.por
dc.contributor.authorCosta, J. B.por
dc.contributor.authorOliveira, J. M.por
dc.contributor.authorCorrelo, V. M.por
dc.contributor.authorReis, R. L.por
dc.contributor.authorCornish, J.por
dc.identifier.citationMaia F. R., David M., Naot D., da silva L., Bastos A. R., Costa J. B., Oliveira J. M., Correlo V. M., Reis R. L., Cornish J. Differentiation of osteoclast precursors on Gellan Gum-based spongy-like hydrogels for bone tissue engineering, Biomedical Materials, Vol. 13, Issue 3, doi:10.1088/1748-605X/aaaf29, 2018por
dc.description.abstractBone tissue engineering with cell-scaffold constructs has been attracting a lot of attention, in particular as a tool for efficient guiding of new tissue formation. However, the majority of the current strategies used to evaluate novel biomaterials focus on osteoblasts and bone formation, while osteoclasts are often overlooked. Consequently, there is limited knowledge about the interaction between osteoclasts and biomaterials. In this study, the ability of gellan gum and hydroxyapatite reinforced gellan gum spongy-like hydrogels to support osteoclastogenesis was investigated in vitro. First, gellan gum and hydroxyapatite reinforced gellan gum spongy-like hydrogels were characterized in terms of microstructure, water uptake and mechanical properties. Then, bone marrow cells isolated from mice long bones and cultured in the spongy-like hydrogels were treated with 1,25-dihydroxyvitamin D3 to promote osteoclastogenesis. It was shown that the addition of HAp to Gellan Gum spongy-like hydrogels enables the formation of lager pores and thicker walls, promoting an increase in stiffness. hydroxyapatite reinforced gellan gum spongy-like hydrogels supported the formation of aggregates of tartrate-resistant acid phosphatase-stained cells and the expression of the genes encoding DC-Stamp and Cathepsin K, suggesting the differentiation of bone marrow cells into pre-osteoclasts. The hydroxyapatite reinforced gellan gum spongy-like hydrogels developed in this work show promise for future use in bone tissue scaffolding applications.por
dc.description.sponsorshipThis work was developed under the scope of the European Project skelGEN (Project No: 318553). F.R. Maia acknowledges ERC-2012-ADG 20120216-321266 (ComplexiTE) for her Postdoc scholarship and Boehringer Ingelheim Fonds for her travel grant. L. P. da Silva acknowledges Portuguese Foundation for Science and Technology (FCT) for her grant (SFRH/BD/78025/2011), J. M. Oliveira thanks FCT for his distinction attributed under the FCT Investigator program (IF/00423/2012 and IF/01285/2015). VM Correlo acknowledges Investigator FCT program (IF/01214/2014).por
dc.publisherIOP Publishingpor
dc.subject3D scaffoldspor
dc.subjectBone Tissue Engineeringpor
dc.subjectspongy-like hydrogelspor
dc.titleDifferentiation of osteoclast precursors on Gellan Gum-based spongy-like hydrogels for bone tissue engineeringpor
dc.subject.wosScience & Technologypor
sdum.journalBiomedical Materialspor
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