Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/56275

TitleDifferentiation of osteoclast precursors on Gellan Gum-based spongy-like hydrogels for bone tissue engineering
Author(s)Maia, F. R.
David, M.
Naot, D.
da silva, L.
Bastos, A. R.
Costa, J. B.
Oliveira, J. M.
Correlo, V. M.
Reis, R. L.
Cornish, J.
Keywords3D scaffolds
Bone Tissue Engineering
hydroxyapatite
Osteoclastogenesis
spongy-like hydrogels
Issue dateFeb-2018
PublisherIOP Publishing
JournalBiomedical Materials
CitationMaia F. R., David M., Naot D., da silva L., Bastos A. R., Costa J. B., Oliveira J. M., Correlo V. M., Reis R. L., Cornish J. Differentiation of osteoclast precursors on Gellan Gum-based spongy-like hydrogels for bone tissue engineering, Biomedical Materials, Vol. 13, Issue 3, doi:10.1088/1748-605X/aaaf29, 2018
Abstract(s)Bone tissue engineering with cell-scaffold constructs has been attracting a lot of attention, in particular as a tool for efficient guiding of new tissue formation. However, the majority of the current strategies used to evaluate novel biomaterials focus on osteoblasts and bone formation, while osteoclasts are often overlooked. Consequently, there is limited knowledge about the interaction between osteoclasts and biomaterials. In this study, the ability of gellan gum and hydroxyapatite reinforced gellan gum spongy-like hydrogels to support osteoclastogenesis was investigated in vitro. First, gellan gum and hydroxyapatite reinforced gellan gum spongy-like hydrogels were characterized in terms of microstructure, water uptake and mechanical properties. Then, bone marrow cells isolated from mice long bones and cultured in the spongy-like hydrogels were treated with 1,25-dihydroxyvitamin D3 to promote osteoclastogenesis. It was shown that the addition of HAp to Gellan Gum spongy-like hydrogels enables the formation of lager pores and thicker walls, promoting an increase in stiffness. hydroxyapatite reinforced gellan gum spongy-like hydrogels supported the formation of aggregates of tartrate-resistant acid phosphatase-stained cells and the expression of the genes encoding DC-Stamp and Cathepsin K, suggesting the differentiation of bone marrow cells into pre-osteoclasts. The hydroxyapatite reinforced gellan gum spongy-like hydrogels developed in this work show promise for future use in bone tissue scaffolding applications.
TypeArticle
URIhttp://hdl.handle.net/1822/56275
DOI10.1088/1748-605X/aaaf29
ISSN1748-605X
Publisher versionhttp://iopscience.iop.org/article/10.1088/1748-605X/aaaf29
Peer-Reviewedyes
AccessRestricted access (UMinho)
Appears in Collections:3B’s - Artigos em revistas/Papers in scientific journals

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