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https://hdl.handle.net/1822/55756
Registo completo
Campo DC | Valor | Idioma |
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dc.contributor.author | Nobre, Clarisse | por |
dc.contributor.author | Gonçalves, D. A. | por |
dc.contributor.author | Teixeira, J. A. | por |
dc.contributor.author | Rodrigues, L. R. | por |
dc.date.accessioned | 2018-09-05T14:45:24Z | - |
dc.date.available | 2018-09-05T14:45:24Z | - |
dc.date.issued | 2018-12 | - |
dc.identifier.citation | Nobre, Clarisse; Gonçalves, D. A.; Teixeira, José A.; Rodrigues, Lígia R., One-step co-culture fermentation strategy to produce high-content fructo-oligosaccharides. Carbohydrate Polymers, 201, 31-38, 2018 | por |
dc.identifier.issn | 0144-8617 | por |
dc.identifier.uri | https://hdl.handle.net/1822/55756 | - |
dc.description.abstract | An integrated process enabling the simultaneous production and purification of fructo-oligosaccharides (FOS) was explored. A co-culture fermentation with Aspergillus ibericus (used as FOS producer strain) and Saccharomyces cerevisiae YIL162W (for small saccharides removal) was optimized. Inoculation conditions of S. cerevisiae, fermentative broth composition, temperature and pH were optimized by experimental design. Yeast extract concentration and temperature were the most significant variables affecting FOS purity. Co-culture fermentations with simultaneously inoculation of the strains, run under 30°C, initial pH 6.0 and 17gL1 yeast extract led to FOS mixtures with 97.4±0.2% (w/w) purity. The fermentations conducted in bioreactor, at a 0.8 vvm aeration rate, yielded 0.70±0.00gFOS.ginitial GF1 at 45h fermentation, with a FOS content of 133.7±0.1gL1. A purity of FOS up to 93.8±0.7% (w/w) was achieved. The one-step fermentation proved to be efficient, economical and fast. | por |
dc.description.sponsorship | Clarisse Nobre and Lígia R. Rodrigues acknowledgethe Portuguese Foundation for Science and Technology (FCT) for the Grants reference SFRH/BPD/87498/2012 and SFRH/BSAB/142873/2018. This study was supported by the FCT under the scope of the strategic funding of UID/BIO/04469/2013 unit and COMPETE 2020 (POCI-01-0145-FEDER-006684) and BioTecNorte operation (NORTE-01-0145-FEDER-000004) funded by the European Regional Development Fund under the scope of Norte2020 Programa Operacional Regional do Norte also, Project ColOsH 02/SAICT/ 2017 (POCI-01-0145-FEDER-030071), MultiBiorefinery SAICTPAC/0040/2015 (POCI-01-0145-FEDER-016403), Biomass and Bioenergy Research Infrastructure 01/SAICT/2016 (PINFRA/22059/2016) and Project FoSynBio 02/SAICT/2017 (POCI-01-0145-FEDER-029549). | por |
dc.language.iso | eng | por |
dc.publisher | Elsevier 1 | por |
dc.relation | info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F87498%2F2012/PT | por |
dc.relation | SFRH/BSAB/142873/2018 | por |
dc.relation | info:eu-repo/grantAgreement/FCT/5876/147337/PT | por |
dc.rights | openAccess | por |
dc.subject | Fructo-oligosaccharides | por |
dc.subject | Co-culture | por |
dc.subject | Integrated process | por |
dc.subject | One-step fermentation | por |
dc.subject | Aspergillus ibericus | por |
dc.subject | Saccharomyces cerevisiae | por |
dc.title | One-step co-culture fermentation strategy to produce high-content fructo-oligosaccharides | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | http://www.journals.elsevier.com/carbohydrate-polymers/ | por |
dc.comments | CEB47910 | por |
oaire.citationStartPage | 31 | por |
oaire.citationEndPage | 38 | por |
oaire.citationConferencePlace | United Kingdom | - |
oaire.citationVolume | 201 | por |
dc.date.updated | 2018-09-04T16:56:11Z | - |
dc.identifier.doi | 10.1016/j.carbpol.2018.08.051 | por |
dc.identifier.pmid | 30241824 | por |
dc.description.publicationversion | info:eu-repo/semantics/publishedVersion | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Carbohydrate Polymers | por |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_47910_1.pdf | 1,15 MB | Adobe PDF | Ver/Abrir |