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TitleSingle-step production of arabino-xylooligosaccharides by recombinant Bacillus subtilis 3610 cultivated in brewers spent grain
Author(s)Amorim, Cláudia
Silvério, Sara Isabel Cruz
Silva, Soraia P.
Coelho, Elisabete
Coimbra, Manuel A.
Prather, Kristala L. J.
Rodrigues, L. R.
KeywordsBrewers spent grain
Bacillus subtilis
Trichoderma reesei
Issue dateNov-2018
JournalCarbohydrate Polymers
CitationAmorim, Cláudia; Silvério, Sara C.; Silva, Soraia P.; Coelho, Elisabete; Coimbra, Manuel A.; Prather, Kristala L. J.; Rodrigues, Lígia R., Single-step production of arabino-xylooligosaccharides by recombinant Bacillus subtilis 3610 cultivated in brewers spent grain. Carbohydrate Polymers, 199, 546-554, 2018
Abstract(s)Brewers spent grain (BSG) is an inexpensive and abundant brewery by-product that can be used to produce prebiotic arabino-xylooligosaccharides (AXOS). In this study, Bacillus subtilis 3610 was used, for the first time, to produce AXOS through direct fermentation of BSG. Additionally, the microorganism was genetically modified to improve the AXOS production. The xylanase gene xyn2 from Trichoderma reesei coupled with a secretion tag endogenous to B. subtilis was cloned in pDR111 and integrated into its chromosome. After optimization by experimental design, AXOS with a degree of polymerization ranging from 2 to 6 were obtained. The maximum production yield expressed in xylose equivalents per amount of BSG (54.2 ± 1.1 mg/g) represents an increase of 33% comparing to the wild type. When compared with the enzymatic hydrolysis process, single-step fermentation with B. subtilis proved to be a very promising low-cost strategy for the simultaneous production of AXOS and valorization of BSG.
Publisher version
AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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